- hec2 (See other available formats)
- Other Names
- CD99, MIC2, HBA71, MSK5X, E2 antigen
- Mouse IgG1, κ
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- Product Citations
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CD99 is a type I single chain transmembrane protein devoid of N-linked glycosylation sites encoded by the pseudoautosomal gene MIC2. CD99 has an apparent molecular weight of 32 kD and is widely expressed on a variety of tissues. CD99 is highly expressed on thymocytes, T cells, and T cell leukemias and lymphomas. However, it is absent on some B cell lines, fetal B cells, eosinophils, granulocytes and the NK-cell line YT. CD99 is involved in spontaneous rosette formation with erythrocytes and may also be involved in other T-cell and hematopoietic cell adhesion pathways. CD99 has been reported to activate a caspase-independent death pathway in T cells under some conditions. CD99 interacts with a number of proteins including ferritin heavy chain 1, karyopherin beta 1, TRIP13, cyclophilin A, annexin II, and ubiquitin-conjugating enzyme E2H.Product Details
- Antibody Type
- Host Species
- Human endothelial cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
Block, IHC, IP, WB - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
(PubMed link indicates BioLegend citation)
- Schenkel AR, et al. 2002. Nat. Immunol. 3:143-50. (IHC, FC, WB)
- Lou O, et al. 2007. J. Immunol. 178:1136-43. (Block, FC)
- Watson RL, et al. 2015. J. Exp. Med. 212:1021-41. (IP, WB)
AB_2814381 (BioLegend Cat. No. 398202)
- Surface glycoprotein, type I transmembrane protein, 32 kD.
T-cells, thymocytes, T-cell leukemias and lymphomas. It is absent on fetal B-cells, eosinophils, granulocytes, and NK cell line YT.
- Leukocyte migration, T-cell adhesion, caspase independent T-cell death, and spontaneous rosette formation with erythrocytes.
- Annexin II, E2H, Ferritin heavy chain 1, karyopherin beta 1, and TRIP13.
- Cell Type
- B cells, Basophils, Dendritic cells, Fibroblasts, Macrophages, Mast cells, NK cells, Platelets, T cells
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
- Gelin C, et al. 1989. EMBO J. 8:3253-59.
- Goodfellow PJ, et al. 1986. Science. 234:740-43.
- Pettersen RD, et al. 2001. J. Immunol. 166:4931-42.
- Gene ID
- 4267 View all products for this Gene ID
- View information about CD99 on UniProt.org
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