Purified anti-human CD99 Antibody

Pricing & Availability
Clone
hec2 (See other available formats)
Other Names
CD99, MIC2, HBA71, MSK5X, E2 antigen
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
hec2_CD99_Antibody_080219
Human peripheral blood lymphocytes were stained with purified anti-human CD99 (clone hec2, filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG PE.
  • hec2_CD99_Antibody_080219
    Human peripheral blood lymphocytes were stained with purified anti-human CD99 (clone hec2, filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG PE.
Cat # Size Price Quantity Avail. Save
398202 100 µg £67
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Description

CD99 is a type I single chain transmembrane protein devoid of N-linked glycosylation sites encoded by the pseudoautosomal gene MIC2. CD99 has an apparent molecular weight of 32 kD and is widely expressed on a variety of tissues. CD99 is highly expressed on thymocytes, T cells, and T cell leukemias and lymphomas. However, it is absent on some B cell lines, fetal B cells, eosinophils, granulocytes and the NK-cell line YT. CD99 is involved in spontaneous rosette formation with erythrocytes and may also be involved in other T-cell and hematopoietic cell adhesion pathways. CD99 has been reported to activate a caspase-independent death pathway in T cells under some conditions. CD99 interacts with a number of proteins including ferritin heavy chain 1, karyopherin beta 1, TRIP13, cyclophilin A, annexin II, and ubiquitin-conjugating enzyme E2H.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human endothelial cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block, IHC, IP, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Schenkel AR, et al. 2002. Nat. Immunol. 3:143-50. (IHC, FC, WB)
  2. Lou O, et al. 2007. J. Immunol. 178:1136-43. (Block, FC)
  3. Watson RL, et al. 2015. J. Exp. Med. 212:1021-41. (IP, WB)
RRID
AB_2814381 (BioLegend Cat. No. 398202)

Antigen Details

Structure
Surface glycoprotein, type I transmembrane protein, 32 kD.
Distribution

T-cells, thymocytes, T-cell leukemias and lymphomas. It is absent on fetal B-cells, eosinophils, granulocytes, and NK cell line YT.

Function
Leukocyte migration, T-cell adhesion, caspase independent T-cell death, and spontaneous rosette formation with erythrocytes.
Interaction
Annexin II, E2H, Ferritin heavy chain 1, karyopherin beta 1, and TRIP13.
Cell Type
B cells, Basophils, Dendritic cells, Fibroblasts, Macrophages, Mast cells, NK cells, Platelets, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Gelin C, et al. 1989. EMBO J. 8:3253-59. 
  2. Goodfellow PJ, et al. 1986. Science. 234:740-43. 
  3. Pettersen RD, et al. 2001. J. Immunol. 166:4931-42.
Gene ID
4267 View all products for this Gene ID
UniProt
View information about CD99 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 08/02/2019

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