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Purified anti-human CD257 (BAFF, BLYS) Antibody

Pricing & Availability
Clone
1D6 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
BAFF, BLYS, TNFSF13b, B cell activating factor, tumor necrosis factor ligand superfamily 13B, B lymphocyte stimulator (BLYS), TNF and APOL-related leukocyte expressed ligand 1 (TALL1), TNF homolog that activates apoptosis, NF-κB and JNK (THANK)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1D6_Purified_CD257_Antibody_FC_021815
Human CD257-transfected bovine coronary artery endothelial cell line (B300) was stained with purified anti-human CD257 (clone 1D6) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), and was then followed by anti-mouse IgG PE.
  • 1D6_Purified_CD257_Antibody_FC_021815
    Human CD257-transfected bovine coronary artery endothelial cell line (B300) was stained with purified anti-human CD257 (clone 1D6) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), and was then followed by anti-mouse IgG PE.
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366502 100 µg £141
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Description

BAFF, also known as B cell activating factor, tumor necrosis factor ligand superfamily 13B, B lymphocyte stimulator (BLYS), TNF and APOL-related leukocyte expressed ligand 1 (TALL1), and TNF homolog, activates apoptosis, NF-κB, and JNK (THANK). BAFF is a type II transmembrane protein and is a member of the tumor necrosis factor ligand superfamily. BAFF can be proteolytically cleaved to form a soluble protein. BAFF, like other TNF ligand family members, forms homotrimers. The predicted molecular weight of a BAFF monomer is approximately 31 kD. BAFF is expressed on peripheral blood B and T lymphocytes, monocytes, macrophages, and dendritic cells. It is upregulated by IFN-γ and downregulated by PMA/ionomycin treatment.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD257 transfected murine cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Hase H, et al. 2004. Blood 6:2257. (IHC)
Product Citations
  1. Majri SS, et al. 2018. J Immunol. 200:110. PubMed
RRID
AB_2565484 (BioLegend Cat. No. 366502)

Antigen Details

Structure
Type II transmembrane protein and is a member of the tumor necrosis factor ligand superfamily. It can be proteolytically cleaved to form a soluble protein and it forms homotrimers. The predicted molecular weight of monomer is approximately 31 kD.
Distribution

Peripheral blood B and T lymphocytes, monocytes, macrophages, and dendritic cells. Upregulated by IFN-γ and is downregulated by PMA/ionomycin treatment.

Function
Stimulates B and T cell immunity, regulates humoral immunity by binding TACI and BCMA receptors, and prevents apoptosis and promotes B cell survival by binding the BAFF-specific receptor (BAFFR/BR3).
Ligand/Receptor
BAFF binds to TACI (TNFRSF13B) and BCMA (TNFRSF17) as well as the APRIL ligand. BAFF also binds to the BAFF-specific receptor (BAFFR/BR3).
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, T cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction, Stem Cells
Molecular Family
CD Molecules, Cytokines/Chemokines, Growth Factors, Soluble Receptors
Antigen References

1. Schneider P, et al. 1999. J. Exp. Med. 189:1747.
2. Moore PA, et al. 1999. Science 285:260.
3. Shu HB, et al. 1999. J. Leuko. Biol. 65:680.
4. Yu G, et al. 2000. Nat. Immunol. 1:252.
5. Oren DA, et al. 2002. Nat. Struct. Biol. 9:288.

Gene ID
10673 View all products for this Gene ID
UniProt
View information about CD257 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/18/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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