Purified anti-human CD1a Antibody

Pricing & Availability
Clone
HI149 (See other available formats)
Regulatory Status
RUO
Workshop
V CD01.01
Other Names
T6, R4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HI149_Purified_053007
Human T lymphoblastic leukemia cell line Molt-4 stained with purified HI149, followed by anti-mouse IgGs FITC
  • HI149_Purified_053007
    Human T lymphoblastic leukemia cell line Molt-4 stained with purified HI149, followed by anti-mouse IgGs FITC
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300101 25 µg £31.00
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300102 100 µg £58.00
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Description

CD1a is a 49 kD member of the immunoglobulin superfamily also known as T6 and R4. It is a type I membrane glycoprotein with structural similarities to MHC class I and is non-covalently associated with β2-microglobulin. CD1a plays a role in non-peptide glycolipid antigen presentation to CD1-restricted T cells. It is expressed on cortical double positive and single positive thymocytes, Langerhans cells, and dendritic cells. In addition to antigen presentation, CD1a has been implicated in thymic T cell development.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Patton KM, et al. 2005. Infect. Immun.73:2083. PubMed
  4. Curti A, et al. 2010. Haematologica. 95:2022. PubMed
Product Citations
  1. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  2. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  3. Eriksson E, et al. 2017. Clin Cancer Res. 5.018055556. PubMed
  4. Guo T, et al. 2018. J Immunol. 200:500. PubMed
  5. Laudanski K, et al. 2018. J Transl Med. 0.765972222. PubMed
  6. Ortiz AM, et al. 2018. Nat Med. 24:1313. PubMed
  7. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  8. Patton K, et al. 2005. Infect Immun. 73:2083. PubMed
  9. Tjomsland V, et al. 2011. PLoS One. 6:e23542. PubMed
  10. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
RRID
AB_314015 (BioLegend Cat. No. 300101)
AB_314016 (BioLegend Cat. No. 300102)

Antigen Details

Structure
Ig superfamily, MHC I-like molecule, type I transmembrane glycoprotein, 49 kD
Distribution

Cortical thymocytes, Langerhans cells, dendritic cells

Function
Antigen presentation, lymphocyte activation, thymic T cell development
Ligand/Receptor
CD1-restricted TCRs
Cell Type
Dendritic cells, Langerhans cells, Thymocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References
  1. Blumberg RS, et al. 1995. Immunol. Rev. 147:5.
  2. Calabi F, et al. 1991. Tissue Antigens 37:1.
  3. Melian A, et al. 1996. Curr. Opin. Immunol. 8:82.
Gene ID
909 View all products for this Gene ID
UniProt
View information about CD1a on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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