Purified anti-human CD147 Antibody

Pricing & Availability
Clone
A20046A (See other available formats)
Other Names
Neurothelin, Basigin, EMMPRIN
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A20046A_PURE_CD147_Anitbody_030821
Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL purified anti-human CD147 (clone A20046A) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with goat anti-mouse secondary antibody (Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
  • A20046A_PURE_CD147_Anitbody_030821
    Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL purified anti-human CD147 (clone A20046A) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with goat anti-mouse secondary antibody (Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
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364602 100 µg £62
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Description

CD147, also known as neurothelin or basigin, is a member of the Ig superfamily. It is a 55-65 kD type I transmembrane glycoprotein which is primarily expressed on leukocytes, erythrocytes, platelets, and endothelial cells. CD147 is reported to have a function during embryonal brain development and/or play a role in integrin-mediated adhesion in brain endothelia.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2890794 (BioLegend Cat. No. 364602)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55-65 kD
Distribution

Leukocytes, erythrocytes, platelets, endothelial cells

Function
Adhesion
Antigen References

1. Biswas C, et al. 1995. Cancer Res. 55:434.
2. Fadool J, et al. 1993. Dev. Dyn. 196:252.
3. Felzmann T, et al. 1991. J. Clin. Immunol. 11:205.

Gene ID
682 View all products for this Gene ID
UniProt
View information about CD147 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 03/08/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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