Purified anti-Haptoglobin Antibody

Pricing & Availability
Clone
BL28630 (See other available formats)
Other Names
Zonulin, Alpha Polypeptide, Beta polypeptide, Binding Peptide, HP2ALPHA2, HPA1S, BP, Haptoglobin Alpha(1S)-Beta, Haptoglobin Alpha(2FS)-Beta
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
BL28630_PURE_Haptoglobin_Antibody_070319.png
Western blot of purified anti-Haptoglobin antibody (clone BL28630). Lane 1: Molecular weight marker; Lane 2: 1 µg of human plasma; Lane 3: 2 µg of human plasma; Lane 4: 5 µg of human plasma; Lane 5: 10 µg of human plasma. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28630_PURE_Haptoglobin_Antibody_070319.png
    Western blot of purified anti-Haptoglobin antibody (clone BL28630). Lane 1: Molecular weight marker; Lane 2: 1 µg of human plasma; Lane 3: 2 µg of human plasma; Lane 4: 5 µg of human plasma; Lane 5: 10 µg of human plasma. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
863001 25 µg £60
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Description

Haptoglobin is a protein that is mainly expressed by hepatic cells in the liver. Haptoglobin is an acute-phase antioxidant that binds to free hemoglobin (Hb) in the plasma and inhibits the oxidative activity of hemoglobin. Individuals carrying the haptoglobin 2-2 genotype have decreased affinity to Hb and are at a higher risk of developing cerebral aneurysm-related complications due to impaired anti-inflammatory pathways. This highlights haptoglobin as part of the Hb scavenging mechanism, which may be critical for clearing Hb after intracranial bleeding and neuroprotection.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Haptoglobin corresponding to amino acids 168 – 391 expressed by E.coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1 - 5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Haptoglobin is a 406 amino acid protein with predicted and observed molecular mass of ~45 kD.
Distribution

Tissue Distribution: Liver and blood plasma
Cellular Distribution: Extracellular and cytosol

Function
Haptoglobin is a part of the hemoglobin scavenging mechanism that clears Hb released into the central nervous system after intracranial bleeding.
Interaction
Haptoglobin forms tetramers of two alpha and two beta chains. It binds to hemoglobin and CD163.
Biology Area
Neurodegeneration, Neuroinflammation, Neuroscience
Molecular Family
Blood Group Markers
Antigen References
  1. Bulters D, et al. 2018. Nat Rev Neurol. 14(7):416.
  2. Blackburn SL, et al. 2018. Front Physiol. 9:592.
  3. Bamm VV, et al. 2017. Metab Brain Dis. 32(1):19-34.
Gene ID
3240 View all products for this Gene ID
UniProt
View information about Haptoglobin on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 07/03/2019

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