Purified anti-Galectin-2 Antibody

Pricing & Availability
Clone
A17160B (See other available formats)
Other Names
LGALS2, HL14, Lectin, CGL-2, Galactoside-Binding, Soluble, 2 Beta-Galactoside-Binding Lectin L-14-II, Lactose-Binding Lectin 2, S-Lac Lectin 2, Gal-2
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A17160B_Pure_Galectin-2_Antibody_1_110118
Western blot of purified anti-Galectin-2 antibody (clone A17160B). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17160B_Pure_Galectin-2_Antibody_1_110118
    Western blot of purified anti-Galectin-2 antibody (clone A17160B). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17160B_Pure_Galectin-2_Antibody_2_110118
    IHC staining of purified anti-Galectin-2 antibody (clone A17160B) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using sodium citrate H.I.E.R, the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
  • A17160B_Pure_Galectin-2_Antibody_3_110118
    IHC staining of purified anti-Galectin-2 antibody (clone A17160B) on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using sodium citrate H.I.E.R, the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
  • A17160B_Pure_Galectin-2_Antibody_4_110118
    IHC staining of purified anti-Galectin-2 antibody (clone A17160B) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using sodium citrate H.I.E.R, the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
  • A17160B_Pure_Galectin-2_Antibody_5_110118
    IHC staining of purified anti-Galectin-2 antibody (clone A17160B) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using sodium citrate H.I.E.R, the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
Cat # Size Price Quantity Avail. Save
856601 25 µg £60
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856602 100 µg £158
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Description

Human Galectin-2 (Gal-2) is a soluble lactose-binding lectin that was initially cloned from a human hepatoma cDNA library, and designated as L-14-II (LGALS2). The galectins are a family of lectins that possess similar carbohydrate binding specificities and conserved consensus sequences. The mammalian galectins are involved in the regulation of inflammation, cell adhesion, cell proliferation, and cell death. Galectins are divided into three groups: prototype, tandem repeat a, and chimera type. Gal-2 belongs to the prototype group that includes Galectins 1, 2, 5, 7, 10, 11, 13, 14, and 15. The prototype galectins contain one carbohydrate recognition domain (CRD) and can form homodimers. Gal-2 shares 43% amino acid sequence identity with Gal-1. Studies in rat tissues and human tumor cell lines have shown that Gal-2 is expressed in gastrointestinal tract. Gal-2 induces apoptosis of lamina propria T lymphocytes, and ameliorates acute and chronic experimental colitis in mice. In addition, Gal-2 and Gal-1 induce phosphatidylserine exposure in fMLP-activated human neutrophils, but not in resting neutrophils. Galectin-1, -2, and -3 exhibit differential recognition of sialylated glycans, and  Gal-2 and Gal-3 possesses higher binding than Gal-1 to fucose-containing A and B blood group antigens

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Galectin-2 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.1 - 1.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783460 (BioLegend Cat. No. 856601)
AB_2783461 (BioLegend Cat. No. 856602)

Antigen Details

Structure
Human Galectin-2 is a 132 amino acid protein with a molecular mass of ~15 kD.
Distribution

Tissue distribution: Highly expressed in gastrointestinal tract, and to lesser extent in the immune system, kidney, and brain.

Cellular distribution: Cytosol.

Function
Galectin-2 his involved in immune cell homeostasis, inflammation, cell adhesion and protein trafficking. The protein levels of Galectin-2, and also other Galectin proteins, are significantly increased in colon and breast cancer patients, promoting cancer cell adhesion.
Interaction
Interactions: Glycans
Cellular targets: Interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α)
Ligand Receptor
CD14, Toll-like receptor (TLR)-4 pathway, glycoconjugates
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules
Antigen References
  1. Delacour D, et al. 2009. Traffic. 10(10):1405-13.
  2. Barrow H, et al. 2011. Clin Cancer Res. 17(22):7035-46.
  3. Rabinovich G.A., et al. 2002. Trends Immunol. 23(6):313-20.
  4. Yıldırım C. et al. 2015. PLoS One. 10(4):e0124347.
Gene ID
3957 View all products for this Gene ID
UniProt
View information about Galectin-2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/02/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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