Purified anti-Fascin-1 Antibody

Pricing & Availability
Clone
CPTC-Fascin1-2 (See other available formats)
Other Names
FASN1, 55 kDa actin-bundling protein, Singed-like protein, p55
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
CPTC-Fascin1-2_PURE_Fascin-1_Antibody_092719.png
Western blot of purified anti-Fascin-1 antibody (clone CPTC-Fascin1-2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of SH-SY5Y cell lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • CPTC-Fascin1-2_PURE_Fascin-1_Antibody_092719.png
    Western blot of purified anti-Fascin-1 antibody (clone CPTC-Fascin1-2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of SH-SY5Y cell lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
868301 25 µg £60
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868302 100 µg £158
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Description

Fascin is a 55 kD F-actin binding protein that is involved in cell motility and migration. Fascin plays an important role in the organization and formation of membrane ruffles, stress fibers, and a diverse set of cell protrusions, such as filopodia. In the mouse subventricular zone-derived migratory neuroblasts, Fascin is highly upregulated to guide the migration of cells along the rostral migratory stream. In Drosophila, Fascin controls neuronal class-specific dendrite arbor morphology to elicit the accurate establishment of neuronal circuits.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Fascin full-length protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Human Fascin is a 493 amino acid protein with a molecular mass of 54 kD.
Distribution

Tissue distribution: Ubiquitously expressed
Cellular distribution: Cytosol, cytoskeleton, and stress fibers

Function
Fascin is involved in the assembly of filamentous actin.
Interaction
Fascin interacts with RUFY3, beta-catenin, and PLXNB3.
Cell Type
Neurons
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neurodegeneration, Neuroscience
Molecular Family
Cytoskeletal Proteins
Antigen References
  1. Yamashiro S, et al. 1998. Mol Biol Cell. 9(5): 993-1006.
  2. Nagel J, et al. 2012. Development 139, 2999-3009
  3. Sonego M, et al. 2013. J Neurosci. 33(30): 12171–85.
Gene ID
6624 View all products for this Gene ID
UniProt
View information about Fascin-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/27/2019

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