Purified anti-FABP1 Antibody

Pricing & Availability
Clone
Poly28601 (See other available formats)
Other Names
FABP1
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly28601_Purified_FABP1_Antibody_1_052119
Western blot of purified anti-FABP1 antibody (Poly28601). Lane 1: Molecular weight marker; Lane 2: 20 µg of human liver lysate; Lane 3: 20 µg of mouse liver lysate; Lane 4: 40 µg of rat liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28601_Purified_FABP1_Antibody_1_052119
    Western blot of purified anti-FABP1 antibody (Poly28601). Lane 1: Molecular weight marker; Lane 2: 20 µg of human liver lysate; Lane 3: 20 µg of mouse liver lysate; Lane 4: 40 µg of rat liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28601_Purified_FABP1_Antibody_2_052119
    IHC staining of purified anti-FABP1 antibody (Poly28601) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • Poly28601_Purified_FABP1_Antibody_3_052119
    IHC staining of purified anti-FABP1 antibody (Poly28601) on formalin-fixed paraffin-embedded mouse liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • Poly28601_Purified_FABP1_Antibody_4_052119
    IHC staining of purified anti-FABP1 antibody (Poly28601) on formalin-fixed paraffin-embedded rat liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
860101 25 µg £64
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Description

Fatty acid binding protein 1 (FABP1) is involved in fatty acid binding, uptake, transport, and metabolism. FABP1 binds to cholesterol, free fatty acids, and bilirubin. Diseases associated with FABP1 include Hepatocellular Adenoma, Fatty Liver Disease and Nonalcoholic 1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant Recombinant Fatty Acid Binding Protein 1 (FABP1) expressed in E.coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
FABP1 is a 127 amino acid protein with an apparent molecular mass of ~ 14 kD.
Distribution

Tissue Distribution: Liver, kidney, colon, rectum
Cellular Distribution: Cytosol and nucleus

Function
FAPB1 is involved in fatty acid uptake, transport, and metabolism.
Interaction
Cholesterol, free fatty acids, bilirubin
Cell Type
Mesenchymal cells
Biology Area
Cell Biology, Cell Proliferation and Viability
Molecular Family
Carrier Proteins
Antigen References
  1. Amiri M, et al. 2018. Gene. 676:171.
  2. Xu Y, et al. 2015. Clin Chim Acta. 445:85.
  3. Thumser AE, et al. 2014. Curr Opin Clin Nutr Metab Care. 17(2):124.
Gene ID
32168 View all products for this Gene ID 14080 View all products for this Gene ID 24360 View all products for this Gene ID
UniProt
View information about FABP1 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 06/17/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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