- E2F1/KH95 (See other available formats)
- Regulatory Status
- Other Names
- PBR3, E2F Transcription Factor 1, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, RBAP-1, RBBP-3, PRB-Binding Protein E2F-1, Transcription Factor E2F1
- Mouse IgG2a, κ
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- Product Citations
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E2F1 is a member of the E2F family of transcription factors, which is comprised of 6 members that display both activator and repressor functions. The primary functions of E2F1 are transcription activation and cell cycle regulation, namely in replication, DNA-damage response and repair, and apoptosis. E2F1 activation during G1 phase of cell cycle is vital for the transition into S phase and the subsequent replication and synthesis of DNA. Beyond its role in cell cycle control, E2F1 is a regulator of metabolism, primarily through β cell proliferation, insulin secretion, endocrine function, and adipogenesis. E2F1 activity is regulated through its binding to phosphorylated retinoblastoma proteins (pRB) and through acetylation when in its pRB-free form.
E2F1 represents a critical target in cancer therapy. Through its stimulation of mTORC1 activity and tumor malignancy and growth, as well as its contribution to the Warburg effect by inducing glycolytic genes, E2F1 often behaves as an oncogene. Additionally, E2F1 can function as a tumor suppressor. Tumor-suppressive properties stem from its role in apoptosis through association with p53 and p73, as well as through inhibition of the NF-κB signaling pathway.
- Antibody Type
- Host Species
- Full-length recombinant human E2F1 protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- The antibody was purified by affinity chromatography.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
IP - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone was tested for ICC using HeLa cells fixed with 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. Neither method resulted in E2F1 staining.
This clone does not recognize mouse E2F1. However, when tested by western blot on mouse lysates, a ~35 kD protein of unknown origin was observed.
AB_2888772 (BioLegend Cat. No. 941101)
AB_2888772 (BioLegend Cat. No. 941102)
- E2F1 is a 437 amino acid protein with a predicted molecular weight of 47 kD.
- Transcription factor/Cell cycle
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Cell Death, DNA Repair/Replication, Transcription Factors
- Molecular Family
- Tumor Suppressors
- Antigen References
- Denechaud P, et al. 2017. Front. Endocrinol. 8:311.
- Liang Y, et al. 2015. Int. J. Oncol. 48:1650.
- Martinez-Balbas M, et al. 2000. Embo J. 19:662.
- Wu Z, et al. 2009. Int. J. Biochem. Cell Biol. 41:2389.
- Gene ID
- 1869 View all products for this Gene ID
- View information about E2F1 on UniProt.org
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