Purified anti-DOPA Decarboxylase (DDC) Antibody

Pricing & Availability
Clone
A17096A (See other available formats)
Other Names
Aromatic-L-Amino-Acid Decarboxylase
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17096A_PURE_DOPA_Antibody_1_090919
Western blot of purified anti-DOPA Decarboxylase (DDC) antibody (clone A17096A). Lane 1: Molecular weight marker; Lane 2: 30 µg of mouse brain lysate; Lane 3: 20 µg of HEK293 cell lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17096A_PURE_DOPA_Antibody_1_090919
    Western blot of purified anti-DOPA Decarboxylase (DDC) antibody (clone A17096A). Lane 1: Molecular weight marker; Lane 2: 30 µg of mouse brain lysate; Lane 3: 20 µg of HEK293 cell lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17096A_PURE_DOPA_Antibody_2_090919
    IHC staining of anti-DOPA Decarboxylase (DDC) antibody (clone A17096A) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A17096A_PURE_DOPA_Antibody_3_090919
    IHC staining of anti-DOPA Decarboxylase (DDC) antibody (clone A17096A) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
869501 25 µg £60
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869502 100 µg £158
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Description

DOPA decarboxylase (DDC) is the enzyme that catalyzes several decarboxylation reactions including the conversion of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-Hydroxytryptophan (5-HTP) to serotonin, and L-tryotophan to tryptamine. DDC is the rate-limiting enzyme in dopamine synthesis in patients treated with L-DOPA and in serotonin synthesis in people treated with 5-HTP, such as those affected by Parkinson’ disease and Depression, respectively. Therefore, DDC is an important therapeutic target for these disorders. DDC deficiency is associated with severe developmental delay, oculogyric crises, and autonomic dysfunction.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human DOPA Decarboxylase (DDC) Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/ml in human and 0.05 - 10 µg/ml for mouse is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814635 (BioLegend Cat. No. 869501)
AB_2814636 (BioLegend Cat. No. 869502)

Antigen Details

Structure
DDC is a 510 amino acid protein with predicted and observed molecular mass of ~54 kD.
Distribution

Tissue Distribution: Kidney, colon, adrenal gland, and brain
Cellular Distribution: Cytosol

Function
DDC catalyzes several decarboxylation reactions in vivo including the conversion of DOPA to dopamine and 5-HTP to serotonin.
Interaction
DDC forms Homodimer.
Cell Type
Endothelial cells, Oligodendrocytes
Biology Area
Neurodegeneration, Neuroinflammation, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, Enzymes and Regulators
Antigen References
  1. Himmelreich N, et al. 2019. Mol Genet Metab. 127(1):12
  2. Guenter J, et al. 2016. Postepy Hig Med Dosw. 70(0):1424
  3. Montioli R, et al. 2016. Curr Drug Metab. 17(5):513
Gene ID
1644 View all products for this Gene ID 13195 View all products for this Gene ID
UniProt
View information about DOPA Decarboxylase on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 09/09/2019

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