Purified anti-Cortactin (CTTN) Antibody

Pricing & Availability
Clone
4F11/CTTN (See other available formats)
Other Names
Amplaxin, Src Substrate Cortactin, Oncogene EMS1
Isotype
Mouse IgG1, λ
Ave. Rating
Submit a Review
Product Citations
publications
4F11_PURE_Cortactin_Antibody_1_062419.png
Western blot of purified anti-Cortactin (CTTN) antibody (clone 4F11/CTTN). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 4F11_PURE_Cortactin_Antibody_1_062419.png
    Western blot of purified anti-Cortactin (CTTN) antibody (clone 4F11/CTTN). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 4F11_PURE_Cortactin_Antibody_2_062419.png
    ICC staining of purified anti-Cortactin (CTTN) antibody (clone 4F11/CTTN) on SH-SY5Y cells. The cells were fixed with 4% PFA, permeabilized with 0.25% Triton X-100, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then stained with 5 µg/mL of the primary antibody for 1 hour at room temperature, followed by incubation with 1 µg/ml of Alexa Fluor® 594 conjugated goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. The cells were co-stained with Alexa Fluor® 488 anti-Tubulin β 3 (TUBB3) antibody (clone TUJ1) at 1:500 dilution. Nuclei were counterstained with Hoechst 33342, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
868101 25 µg £60
Check Availability


Need larger quantities of this item?
Request Bulk Quote
868102 100 µg £158
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Cortactin is a cytosolic protein that promotes polymerization, rearrangement of the actin cytoskeleton, and synaptogenesis. Stimuli such as alterations in the concentration of growth factors lead to phosphorylation of cortactin by tyrosine or serine/threonine kinases. This modification enables cortactin to bind to filamentous actin and Arp2/3, resulting in the branching of the actin cytoskeleton. In addition, cortactin may be involved in clathrin-independent endocytosis.  Amplification of the cortactin gene is observed in certain tumors, indicating that it may also be a component of tumorigenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. For immunocytochemistry, a concentration range of 2 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Cortactin is a 634 amino acid protein with predicted molecular mass of ~ 71 kD and observed molecular mass of ~ 80 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Plasma membrane, cytoskeleton, cytosol, and Golgi apparatus

Function
Cortactin promotes polymerization and the rearrangement of the actin cytoskeleton upon external stimulations.
Interaction
SHANK2, SHANK3, FGD1, ABL2, KCNA2, PLXDC2, SRCIN1, SMASN1, ASAP1.
Cell Type
Astrocytes, Endothelial cells, Neurons, Oligodendrocytes
Biology Area
Cancer Biomarkers, Cell Motility/Cytoskeleton/Structure, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance, Synaptic Biology
Molecular Family
Cytoskeletal Proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Ramos-Garcia P, et al. 2019. Oral Dis. 25(4):949
  2. Schnoor M, et al. 2018. Trends Cell Biol. 28(2):79
  3. Yin M, et al. 2017. Oncotarget. 8(50):88232
Gene ID
2017 View all products for this Gene ID 13043 View all products for this Gene ID 60465 View all products for this Gene ID
UniProt
View information about Cortactin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/24/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account