Purified anti-Cofilin 1 Antibody

Pricing & Availability
Clone
A18040J (See other available formats)
Other Names
Cofilin 1, CFL1, CFL
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A18040J_Purified_Cofilin-1_Antibody_1_051019.png
Western blot of purified anti-Cofilin 1 antibody (clone A18040J). Lane 1: Molecular weight marker; Lane 2: 20µg of mouse brain lysate; Lane 3: 40µg of rat brain lysate; Lane 4: 10µg of HeLa cell lysate; Lane 5: 10 µg of NIH3T3 cell lysate. The blots were incubated with 5 µg/mL of clone A8040J or mouse IgG overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18040J_Purified_Cofilin-1_Antibody_1_051019.png
    Western blot of purified anti-Cofilin 1 antibody (clone A18040J). Lane 1: Molecular weight marker; Lane 2: 20µg of mouse brain lysate; Lane 3: 40µg of rat brain lysate; Lane 4: 10µg of HeLa cell lysate; Lane 5: 10 µg of NIH3T3 cell lysate. The blots were incubated with 5 µg/mL of clone A8040J or mouse IgG overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18040J_Purified_Cofilin-1_Antibody_2_051019.png
    IHC staining of purified anti-Cofilin 1 antibody (clone A18040J) on formalin-fixed paraffin-embedded mouse colon tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • A18040J_Purified_Cofilin-1_Antibody_3_051019.png
    IHC staining of purified anti-Cofilin 1 antibody (clone A18040J) on formalin-fixed paraffin-embedded rat colon tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
865301 25 µg £60
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865302 100 µg £158
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Description

Cofilin 1 is a ubiquitously expressed actin-regulating protein that binds to and depolymerizes filamentous actin (F-actin), as well as inhibits G-actin polymerization in a pH-dependent mechanism. Cofilin 1 plays a major role in cell migration, particularly tumor cell migration and invasion. A recent study using colorectal cancer as model, compared expression of cofilin 1 in cancer tissue and adjacent non-tumor tissue and suggested that it may be used as a novel diagnostic tumor marker in various cancers4.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CFL1 recombinant protein (a.a. Met 1 - Leu 166)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 10 µg per ml. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For WB on brain lysates, 5 – 10 ug/mL is recommended.

Antigen Details

Structure
Cofilin 1 is a 166 amino acid protein with an apparent molecular mass of ~19 kD.
Distribution

Tissue Distribution: Immune system, gastrointestinal tract, lung, testis, and skin
Cellular Distribution: Nucleus, cytosol, plasma membrane, cytoskeleton, and mitochondrion

Interaction
Cofilin 1 plays a role in cytoskeleton dynamics by regulating F-actin depolimerization
Ligand/Receptor
HSPH1, LIMK1, F-actin
Cell Type
Astrocytes, Dopaminergic Neurons, GABAergic Neurons, Glutamatergic Neurons, Mature Neurons, Microglia, Neural Stem Cells, Neurons, Oligodendrocytes
Biology Area
Cancer Biomarkers, Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Cell Structure, Neurodegeneration, Neuroscience, Synaptic Biology
Molecular Family
Cytoskeletal Proteins, GTPases, Scaffold Proteins
Antigen References
  1. Kanellos G and Frame MC. 2016. J Cell Sci. 129(17):3211
  2. Walsh KP, et al. 2014. Prion. 8(6):375
  3. Xu P, et al. 2018. Front Plant Sci. 9:1528
  4. Mousavi S, et al. 2018. J Gastrointest Oncol. 9(5):791
Gene ID
1072 View all products for this Gene ID 12631 View all products for this Gene ID 29271 View all products for this Gene ID
UniProt
View information about Cofilin 1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/10/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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