Purified anti-Clathrin Light Chain (CLTA) Antibody (Previously Covance catalog# MMS-423P)

Pricing & Availability
Clone
CON.1 (See other available formats)
Other Names
Clathrin light chain A, clathrin, light polypeptide (Lca)
Previously
Covance Catalog# MMS-423P
Isotype
Mouse IgG2b
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Product Citations
publications
CONdot1_Pure_CLTA_Antibody_1_102318
IHC staining of purified anti-Clathrin Light Chain (CLTA) antibody (clone CON.1) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • CONdot1_Pure_CLTA_Antibody_1_102318
    IHC staining of purified anti-Clathrin Light Chain (CLTA) antibody (clone CON.1) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • CONdot1_Pure_CLTA_Antibody_2_102318
    IHC staining of purified anti-Clathrin Light Chain (CLTA) antibody (clone CON.1) on formalin-fixed paraffin-embedded human prostate tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • CONdot1_Pure_CLTA_Antibody_3_102318
    ICC staining of purified anti-Clathrin Light Chain (CLTA) antibody (clone CON.1) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 1 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 20X objective. Scale bar: 50 µm
  • CONdot1_Pure_CLTA_Antibody_4_102318
    ICC staining of purified anti-Clathrin Light Chain (CLTA) antibody (clone CON.1) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 1 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Cells were counterstained with Flash Phalloidin™ Green 488 (Cat. No. 424201) at a 1:100 dilution for 20min at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 20X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
811501 100 µL £178
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Description

Clathrin consists of three heavy chains (192kD). The three light chains (LCa or LCb, 25-29kD) self-assembles into a polyhedral cytoskeletal element. The self-assembly facilitates clathrin's central role in membrane sorting and selective transport within the cell.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a peptide derived from bovine clathrin light chain corresponding to the conserved region of the light chains, residues 23-44.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
Store frozen (-20 or -80°C); shipped on blue ice. Aliquot upon receipt before freezing. Avoid repeated freeze/thaw cycles.
Application

IHC-P - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/ml is suggested. For immunocytochemistry, a concentration range of 1.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Zlatic SA, et al. 2013. Mol Biol Cell. 24:2378-88.
  2. Jiang R, et al. 2000. J Biol Chem. 275(12):8439-47.
  3. Nathke IS, et al. 1992. Cell. 68:899-910.
  4. Jackson AP, et al. 1987. Nature. 326:154-9.
RRID
AB_2564758 (BioLegend Cat. No. 811501)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Gene ID
1211 View all products for this Gene ID
UniProt
View information about Clathrin Light Chain (CLTA) on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 10/23/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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