Purified anti-CKMT2 Antibody

Pricing & Availability
Clone
3F4-G5-H5 (See other available formats)
Other Names
Creatine Kinase, Mitochondrial 2
Isotype
Mouse IgG2b, κ
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Product Citations
publications
3F4-G5-H5_PURE_CKMT2_Antibody_1_080219
Western blot of purified anti-CKMT2 antibody (clone 3F4-G5-H5). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 3F4-G5-H5_PURE_CKMT2_Antibody_1_080219
    Western blot of purified anti-CKMT2 antibody (clone 3F4-G5-H5). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 3F4-G5-H5_PURE_CKMT2_Antibody_2_080219
    IHC staining of purified anti-CKMT2 antibody (clone 3F4-G5-H5) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
868701 25 µg £60
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868702 100 µg £158
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Description

CKMT2 is an isoform of the mitochondrial specific creatine kinase subunit that is mainly expressed in skeletal and heart muscle cells. CKMT2 exists in two different oligomeric forms: dimers and octamers. Recent studies have shown that the homooctameric form of CKMT2 is the predominant form in vivo and is critical to its function.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
CKMT2 is a 419 amino acid protein with predicted and observed molecular mass of ~47 kD.
Distribution

Tissue Distribution: Mainly expressed in heart and skeletal muscle. Also expressed in brain.
Cellular Distribution: Mitochondrion and cytosol

Function
CKMT2 plays a central role in energy transduction in tissues with large, fluctuating energy demands such as the brain.
Interaction
CKMT2 exists as octamer composed of four homodimers.
Biology Area
Mitochondrial Function, Neurodegeneration, Neuroscience
Molecular Family
Mitochondrial Markers
Antigen References
  1. Zervou S, et al. 2017. PLoS One. 12(8):e0182994
  2. Forsey KE, et al. 2013. Mol Reprod Dev. 80(3):185
  3. Miceli MV, et al. 2005. Exp Cell Res. 302 (2):270
Gene ID
1160 View all products for this Gene ID 76722 View all products for this Gene ID 688698 View all products for this Gene ID
UniProt
View information about CKMT2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 08/02/2019

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