Purified anti-CD33 Antibody (Previously Covance catalog# MMS-5090)

Pricing & Availability
Clone
P67.6 (See other available formats)
Regulatory Status
RUO
Other Names
Myeloid cell surface antigen CD33, CD33 antigen (gp67), sialic acid binding Ig-like lectin 3,gp67, SIGLEC3, Siglec3
Previously
Covance Catalog# MMS-5090
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P67.6_Purified_CD33_Antibody_IHC_032515
IHC staining of purified anti-CD33 antibody (clone P67.6) on frozen human spleen tissue. The tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • P67.6_Purified_CD33_Antibody_IHC_032515
    IHC staining of purified anti-CD33 antibody (clone P67.6) on frozen human spleen tissue. The tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
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825601 100 µL £177
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Description

CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against FMY9S5 cells containing the CD33 gene.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-F - Quality tested
FC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 10.0 - 20.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunohistochemical staining on frozen tissue (IHC-F) and flow cytometry (FC).

Application References

(PubMed link indicates BioLegend citation)
  1. Favaloro EJ, et al. 1987. Dis. Markers. 5:215. [clone characterization]
  2. Andrews RG, et al. 1983. Blood. 62:124. (FC)
  3. van der Jagt RH, et al. 1992. Cancer Res. 52:89. (FC)
  4. Liesz A, et al. 2015. J. Neurosci. 35:583. (FC)
Product Citations
  1. Tavazoie MF, et al. 2018. Cell. 172:825. PubMed
  2. Melo-Gonzalez F, et al. 2018. J Biol Chem. 293:8543. PubMed
  3. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
RRID
AB_2564890 (BioLegend Cat. No. 825601)

Antigen Details

Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Siglec Molecules
Gene ID
945 View all products for this Gene ID
UniProt
View information about CD33 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 04/02/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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