Purified anti-CAMLG Antibody

Pricing & Availability
Clone
Poly28606 (See other available formats)
Other Names
Calcium signal-modulating cyclophilin ligand, CAML, Calcium Modulating Ligand, Cyclophilin B-Binding Protein, GET2
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly28606_PURE_CAMLG_Antibody_1_062519
Western blot of purified anti-CAMLG antibody (Poly28606). Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain lysate; Lane 3: 20 μg of mouse brain lysate; Lane 4: 20 μg of rat brain lysate. The blot was incubated with 0.05 μg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28606_PURE_CAMLG_Antibody_1_062519
    Western blot of purified anti-CAMLG antibody (Poly28606). Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain lysate; Lane 3: 20 μg of mouse brain lysate; Lane 4: 20 μg of rat brain lysate. The blot was incubated with 0.05 μg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28606_PURE_CAMLG_Antibody_2_071619_updated
    IHC staining of purified anti-CAMLG antibody (Poly28606) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28606_PURE_CAMLG_Antibody_3_062519
    IHC staining of purified anti-CAMLG antibody (Poly28606) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28606_PURE_CAMLG_Antibody_4_062519
    IHC staining of purified anti-CAMLG antibody (Poly28606) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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860601 25 µg £64
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Description

Calcium signal-modulating cyclophilin ligand (CAMLG) is a ubiquitously expressed protein implicated in EGF signaling and EGF receptor (EGFR) trafficking. Deletion of CAMLG gene causes embryonic lethality, suggesting that CAMLG protein functions in early development. Loss of CAMLG leads to defects in the recycling of internalized EGFR to the plasma membrane and reduced cell surface expression without affecting receptor internalization. In the brain, CAMLG regulates the accumulation of GABA receptors at GABAergic synapses. In CAMLG mutant neurons, deficits in the recycling of endocytosed GABA receptors leads to their reduced surface expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant CAMLG (Gly19-Glu184) with two N-terminal tags.
Formulation
0.01M PBS, pH 7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.1 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested for human and a concentration range of 1.0 - 10 µg/ml is suggested for mouse and rat. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Human CAMLG is a 296 amino acid protein with a predicted molecular mass of 33 kD.
Distribution

Tissue distribution: Ubiquitous. Highest level in brain, testis, and ovary.
Cellular distribution: Membrane

Function
CAMLG is involved in EGF signaling and EGFR recycling to cell surface.
Interaction
Interacts with TNFRSF13B/TACI, EGFR, and GABA receptors.
Cell Type
GABAergic Neurons, Lymphocytes, Neurons
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Neurotransmitter Receptor Trafficking/Endocytosis, Postsynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Tran DD, et al. 2003. Dev. Cell. 5:245-56.
  2. Yuan X, et al. 2008. Mol. Cell. Neurosci. 2008.03.002.
Gene ID
819 View all products for this Gene ID 18508 View all products for this Gene ID 25509 View all products for this Gene ID
UniProt
View information about CAMLG on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 07/15/2019

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