Purified anti-Aldose Reductase (AKR1B1) Antibody

Pricing & Availability
Clone
4G9-B6-F6 (See other available formats)
Other Names
Aldo-keto reductase family 1 member B1, Aldehyde reductase, Aldose reductase, AR
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
4G9-B6-F6_PURE_AKR1B1_Antibody_1_111919
Western blot of purified anti-Aldose Reductase (AKR1B1) antibody (clone 4G9-B6-F6). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 4G9-B6-F6_PURE_AKR1B1_Antibody_1_111919
    Western blot of purified anti-Aldose Reductase (AKR1B1) antibody (clone 4G9-B6-F6). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 4G9-B6-F6_PURE_AKR1B1_Antibody_2_111919
    IHC staining of purified anti-Aldose Reductase (AKR1B1) antibody (clone 4G9-B6-F6) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4G9-B6-F6_PURE_AKR1B1_Antibody_3_111919
    IHC staining of purified anti-Aldose Reductase (AKR1B1) antibody (clone 4G9-B6-F6) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4G9-B6-F6_PURE_AKR1B1_Antibody_4_111919
    IHC staining of purified anti-Aldose Reductase (AKR1B1) antibody (clone 4G9-B6-F6) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4G9-B6-F6_PURE_AKR1B1_Antibody_5_111919
    IHC staining of purified anti-Aldose Reductase (AKR1B1) antibody (clone 4G9-B6-F6) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat No. 405326) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4G9-B6-F6_PURE_AKR1B1_Antibody_6_111919
    IHC staining of purified anti-Aldose Reductase (AKR1B1) antibody (clone 4G9-B6-F6) on formalin-fixed paraffin-embedded human placenta tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat No. 405326) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
870301 25 µg £62
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870302 100 µg £161
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Description

Aldo-keto reductase 1 member B1 (AKR1B1) is a member of AKR superfamily and catalyzes the NADPH-dependent reduction of a wide range of carbonyl-containing compounds to their corresponding metabolites. It also plays an important role in glucose metabolism. Elevated expression levels of AKR1B1 correlate with breast cancer, neuroinflammation, and astrocyte proliferation via modulating energy metabolism.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant human AKR1B1 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.2 - 5.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Human aldose reductase is a 316 amino acid protein with a predicted molecular mass of 36 kD.
Distribution

Tissue distribution: Expressed in a wide range of tissues
Cellular distribution: Nucleus, cytosol, extracellular, mitochondrion, lysosome

Function
AKR1B1 plays an important role in cellular metabolism.
Cell Type
Astrocytes, Neurons
Biology Area
Neuroinflammation, Neuroscience
Molecular Family
Enzymes and Regulators
Antigen References
  1. Chen X, et al. 2018. Neurochem Res. 43(8):1491-1499.
  2. Steinhauser CB, et al. 2016. Biol Reprod. 95(5):108.
  3. Wu X, et al. 2017. J Exp Med. 214(4):1065-1079.
  4. Han J, et al. 2016. Proc Natl Acad Sci U S A. 113(50):14283-14288.
Gene ID
231 View all products for this Gene ID
UniProt
View information about Aldose Reductase on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/19/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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