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PerCP/Cyanine5.5 anti-human CD8b Antibody

Pricing & Availability
Clone
S21011A (See other available formats)
Regulatory Status
RUO
Other Names
Lyt3, Ly3, P37, LEU2, CD8B1, CD8b, CD8beta
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
S21011A_PerCP-Cyanine55_-CD8b_Antibody_071823
Human peripheral blood lymphocytes were stained with anti-human CD3 FITC and anti-human CD8b (clone S21011A) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
  • S21011A_PerCP-Cyanine55_-CD8b_Antibody_071823
    Human peripheral blood lymphocytes were stained with anti-human CD3 FITC and anti-human CD8b (clone S21011A) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
387309 25 tests £86
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387310 100 tests £216
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Description

CD8b or CD8β antigen is also known as Lyt3, or Ly-3. It is a member of the immunoglobulin superfamily expressed on most thymocytes, and some mature T cell subsets. CD8β forms heterodimers with the CD8α chain (CD8a). CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase LCK (p56lck).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Immunogen
Human CD8α/CD8β-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. 

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone S21011A does not interfere with the binding of CD8α binding antibodies (SK1, HIT8a, RPA-T8).

Clone S21011A shows comparable staining signals on 4% paraformaldehyde-fixed cells and unfixed cells.

Based on in-house testing, clone S21011A clone reacts with an epitope that is exclusively present on human CD8b, whereas clone QA20A40 reacts with an epitope formed by a heterodimer of human CD8a and CD8b chains.

RRID
AB_3068210 (BioLegend Cat. No. 387309)
AB_3068210 (BioLegend Cat. No. 387310)

Antigen Details

Structure
Ig superfamily
Distribution

Subset of T Cells, most thymocytes

Function
TCR co-receptor
Interaction
CD8α
Ligand/Receptor
MHC Class 1 molecules
Cell Type
Lymphocytes, T cells, Thymocytes
Biology Area
Adaptive Immunity, Cancer Biomarkers, Cell Biology, Immunology
Molecular Family
CD Molecules, TCRs
Antigen References
  1. Johnson P. 1987. Immunogenetics. 26:174-7.
  2. Norment AM & Littman DR, 1988. EMBO J. 7:3433-9.
Gene ID
926 View all products for this Gene ID
UniProt
View information about CD8beta on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 07/18/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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