PE/Dazzle™ 594 anti-human CD2 Antibody

Pricing & Availability
Clone
RPA-2.10 (See other available formats)
Workshop
IV T085
Other Names
LFA-2, T11, SRBC-R
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
RPA-2dot10_PEDZL594_CD2_Antibody_121018
Human peripheral blood lymphocytes stained with CD2 (clone RPA-2.10) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram).
  • RPA-2dot10_PEDZL594_CD2_Antibody_121018
    Human peripheral blood lymphocytes stained with CD2 (clone RPA-2.10) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram).
See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Avail. Save
300227 25 tests £91
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300228 100 tests £175
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Description

CD2 is a 50 kD type I transmembrane glycoprotein also known as LFA-2, T11, and sheep red blood cell receptor (SRBC-R). This immunoglobulin superfamily member is expressed on thymocytes, T lymphocytes, NK cells, and thymic B cell subsets. The major ligand for CD2 is CD58 (also known as LFA-3). CD2 has also been reported to bind CD48, CD59, and CD15. CD2 plays a critical role in alternative T cell activation, T cell signaling, and cell-cell adhesion.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus, Swine (Pig, Porcine)
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions. The solution is free of unconjugated PE/Dazzle™ 594 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6 and blocking of T cell activation2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 300212).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Aversa G, et al. 1987. Transplant. Proc. 19:277. (Block)
  3. Zaretsky AG, et al. 2009. J. Exp Med. 206:991. (IHC) PubMed
  4. Perona-Wright G, et al. 2010. Nat. Immunol. 11:520. (FC) PubMed
  5. Thummler K, et al. 2010. J. Leukoc. Biol. 88:1041.
  6. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
RRID
AB_2783149 (BioLegend Cat. No. 300227)
AB_2783150 (BioLegend Cat. No. 300228)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 50 kD
Distribution

T cells, NK cells, thymocytes, and thymic B cell subsets

Function
T cell activation, adhesion
Ligand Receptor
CD58 (LFA-3), CD48, CD59, CD15
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Bell G, et al. 1995. J. Immunol. 155:2805.
2. Bierer B, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Moingeon P, et al. 1989. Immunol. Rev. 111:111.

Gene ID
914 View all products for this Gene ID
UniProt
View information about CD2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/10/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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