PE/Dazzle™ 594 anti-mouse/rat CD61 Antibody

Pricing & Availability
Clone
2C9.G2 (HMβ3-1) (See other available formats)
Other Names
β3 integrin, Integrin β3 chain, gpIIIa
Isotype
Armenian Hamster IgG
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Product Citations
publications
2C9dotG2_PEDazzle594_CD61_Antibody_070819
C57BL/6 mouse bone marrow cells were stained with CD61 (clone 2C9.G2 (HMβ3-1)) PE/Dazzle™ 594 (filled histogram) or Armenian hamster IgG PE/Dazzle™ 594 isotype control (open histogram).
  • 2C9dotG2_PEDazzle594_CD61_Antibody_070819
    C57BL/6 mouse bone marrow cells were stained with CD61 (clone 2C9.G2 (HMβ3-1)) PE/Dazzle™ 594 (filled histogram) or Armenian hamster IgG PE/Dazzle™ 594 isotype control (open histogram).
See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Avail. Save
104321 25 µg £87
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104322 100 µg £205
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Description

CD61 is a 110 kD integrin β chain also known as β3 integrin or gpIIIa. It associates with the integrin αv chain (CD51) to form the vitronectin receptor. In addition, CD61 can associate with the integrin αIIb chain (CD41) to form the gpIIb/IIIa complex. CD61 is expressed on platelets, megakaryocytes, endothelium, smooth muscle, a subset of B cells, myeloid cells, osteoclasts, and mast cells. CD61, in conjunction with CD41 or CD51, mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. Leukocyte-endothelial adhesion is mediated by the binding of αv3 integrin or vitronectin receptor to CD31 (PECAM-1).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Vitronectin receptor protein from the mouse T-cell hybridoma 2B4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions. The solution is free of unconjugated PE/Dazzle™ 594 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of ligand binding1-4, activation of αv β3 integrin signaling5, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 104310).

Application References

(PubMed link indicates BioLegend citation)
  1. Kieffer N, et al. 1990. Annu. Rev. Cell Biol. 6:329. (Block)
  2. Piali L, et al. 1995. J. Cell Biol. 130:451. (Block)
  3. Ashkar S, et al. 2000. Science 287:860. (Block)
  4. Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block)
  5. Moulder K, et al. 1991. J. Exp. Med. 173:343. (Activ)
  6. Carlson TR, et al. 2008.135:2193. PubMed
  7. Yamaji D, et al. 2009. Genes Dev. 23:2382. PubMed

Antigen Details

Structure
Integrin family, 110 kD
Distribution

Platelets, megakaryocytes, endothelium, smooth muscle, activated T cells, subset of B cells, monocytes, macrophages, osteoclasts, mast cells

Function
Attachment to matrix
Ligand/Receptor
Fibrinogen, von Willebrand factor, fibronectin
Cell Type
B cells, Endothelial cells, Macrophages, Mast cells, Megakaryocytes, Monocytes, Osteoclasts, Platelets, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Phillips DR, et al. 1991. Cell 65:359.
3. Felding-Habermann B, et al. 1993. Curr. Opinion Cell Biol. 5:864.

Gene ID
16416 View all products for this Gene ID 29302 View all products for this Gene ID
UniProt
View information about CD61 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 07/08/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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