- A2A9/6 (See other available formats)
- Other Names
- Mouse IgG2a, κ
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CD41/CD61, also known as gpIIb/IIIa, is a member of a family integrin receptors. This is a complex comprised by CD41 and CD61 through non-covalent association. CD41/CD61 is mainly expressed by platelets and megakaryocytes. The resting form of the CD41/CD61 complex is involved in platelet activation and aggregation by binding to immobilized fibrinogen. After activation, CD41/CD61 becomes a receptor for soluble fibrinogen and several other RGD-containing adhesive proteins such as von Willebrand Factor (vWF) and fibronectin. An absence or dysfunction of CD41/CD61 on the platelet surface results in an inherited bleeding disorder, called Glanzmann Thromsasthenia (GT). CD41/CD61 has been found on murine hematopoietic progenitor cells, indicating that this complex may play a role in regulating hematopoietic development.Product Details
- Antibody Type
- Host Species
- Human platelets
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions. The solution is free of unconjugated PE/Dazzle™ 594 and unconjugated antibody.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for relevant formats) include: immunoprecipitation1 and blocking (aggregation)2. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 359804).
A2A9/6 is recognized as having high affinity to gpIIb/IIIa. It has been shown to inhibit platelet aggregation induced by a variety of agonists and the initiation of clot formation.
(PubMed link indicates BioLegend citation)
- Basani RB, et al. 1996. Blood. 88:167. (IP)
- Bennett JS, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:2417. (Block)
AB_2783265 (BioLegend Cat. No. 359817)
AB_2783266 (BioLegend Cat. No. 359818)
- Complex of CD41 and CD61 through non-covalent association.
Platelets and megakaryocytes.
- Mediates attachment to cells of diverse matrix proteins.
- Ligand Receptor
- Fibrinogen, RGD-containing adhesive proteins such as vonWillebrand Factor (vWF) and fibronectin.
- Cell Type
- Megakaryocytes, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Matsumura-Takeda K, et al. 2007. Stem Cell. 25:862.
2. Corbel C, et al. 2005. 49:279.
3. Bennett JS, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:2417.
4. Clemetson KJ, et al. 1994. Curr. Opin. Hematol. 1:388.
- Gene ID
- 3690 View all products for this Gene ID 3674 View all products for this Gene ID
- View information about CD41/CD61 on UniProt.org
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