PE/Dazzle™ 594 anti-human CD41/CD61 Antibody

Pricing & Availability
Clone
A2A9/6 (See other available formats)
Other Names
gpIIb/gpIIIa
Isotype
Mouse IgG2a, κ
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Product Citations
publications
A2A9-6_PEDZL594_CD41-CD61_Antibody_101018
Human platelets were stained with CD41/CD61 (clone A2A9/6) PE/Dazzle™ 594 (filled histogram) or mouse IgG2a, κ PE/Dazzle™ 594 isotype control (open histogram).
  • A2A9-6_PEDZL594_CD41-CD61_Antibody_101018
    Human platelets were stained with CD41/CD61 (clone A2A9/6) PE/Dazzle™ 594 (filled histogram) or mouse IgG2a, κ PE/Dazzle™ 594 isotype control (open histogram).
See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Avail. Save
359817 25 tests £97
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359818 100 tests £215
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Description

CD41/CD61, also known as gpIIb/IIIa, is a member of a family integrin receptors. This is a complex comprised by CD41 and CD61 through non-covalent association. CD41/CD61 is mainly expressed by platelets and megakaryocytes. The resting form of the CD41/CD61 complex is involved in platelet activation and aggregation by binding to immobilized fibrinogen. After activation, CD41/CD61 becomes a receptor for soluble fibrinogen and several other RGD-containing adhesive proteins such as von Willebrand Factor (vWF) and fibronectin. An absence or dysfunction of CD41/CD61 on the platelet surface results in an inherited bleeding disorder, called Glanzmann Thromsasthenia (GT). CD41/CD61 has been found on murine hematopoietic progenitor cells, indicating that this complex may play a role in regulating hematopoietic development.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human platelets
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions. The solution is free of unconjugated PE/Dazzle™ 594 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1 and blocking (aggregation)2. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 359804).

A2A9/6 is recognized as having high affinity to gpIIb/IIIa. It has been shown to inhibit platelet aggregation induced by a variety of agonists and the initiation of clot formation.

Application References

(PubMed link indicates BioLegend citation)
  1. Basani RB, et al. 1996. Blood. 88:167. (IP)
  2. Bennett JS, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:2417. (Block)
RRID
AB_2783265 (BioLegend Cat. No. 359817)
AB_2783266 (BioLegend Cat. No. 359818)

Antigen Details

Structure
Complex of CD41 and CD61 through non-covalent association.
Distribution

Platelets and megakaryocytes.

Function
Mediates attachment to cells of diverse matrix proteins.
Ligand Receptor
Fibrinogen, RGD-containing adhesive proteins such as vonWillebrand Factor (vWF) and fibronectin.
Cell Type
Megakaryocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Matsumura-Takeda K, et al. 2007. Stem Cell. 25:862.
2. Corbel C, et al. 2005. 49:279.
3. Bennett JS, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:2417. 
4. Clemetson KJ, et al. 1994. Curr. Opin. Hematol. 1:388.

Gene ID
3690 View all products for this Gene ID 3674 View all products for this Gene ID
UniProt
View information about CD41/CD61 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/10/2018

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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