PE anti-mouse Ly-49AB6 Antibody

Pricing & Availability
Clone
A1/Ly49A (See other available formats)
Regulatory Status
RUO
Other Names
Ly49A
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
A1slashLy49A_PE_060210
C57BL/6 mouse splenocytes stained with A1/Ly49A PE and NK1.1 APC
  • A1slashLy49A_PE_060210
    C57BL/6 mouse splenocytes stained with A1/Ly49A PE and NK1.1 APC
See PE spectral data
Cat # Size Price Quantity Check Availability Save
138703 25 µg £59
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Ly-49A is a 44 kD member of the C-type lectin/Ly-49 family proteins. It is a type-II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains. Ly-49A is present on a subset of NK and NK-T cells. Ly-49A, like other members of this family, inhibits NK cell activation through the recognition and interaction of certain MHC class I haplotypes on potential target cells. The Ly-49A receptor recognizes H-2Dd and H-2Dk, triggering phosphorylation of the ITIM motif in the cytoplasmic domain and recruitment of SHP-1 and SHP-2. The A1/Ly49A antibody preferentially reacts with CRD of Ly-49AB6 alloantigen and has also shown cross-reaction with Ly-49ANOD, Ly-49PNOD and Ly-49P129/J.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse C57BL/6N T lymphoma EL-4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Nagasawa R, et al. 1987. J. Immunol. 138:815. (IP)
  2. Guy-Grand D, et al. 1996. Eur. J. Immunol. 26:2248. (FC)
  3. Zimmer J, et al. 2001. J. Exp. Med. 194:1531. (FC)
Product Citations
  1. Wagner JA, et al. 2020. Cell Rep. 31:107720. PubMed
  2. Kissiov DU, et al. 2022. Elife. 11:. PubMed
RRID
AB_2134787 (BioLegend Cat. No. 138703)

Antigen Details

Structure
C-type lectin, Ly-49 family, ITIM, 44 kD
Distribution

NK cells, NKT cells

Function
Inhibitory NK cell receptor
Ligand/Receptor
H-2Dd, H-2Dk, H-2Dp
Cell Type
NK cells, NKT cells
Biology Area
Immunology
Molecular Family
MHC Antigens
Antigen References

1. Coles MC, et al. 2000. Eur. J. Immunol. 30:236.
2. Takei FJ, et al. 1997. Immunol. Rev. 155:67.
3. Hanke T, et al. 1999. Immunity 11:67.
4. Silver ET, et al. 2000. J. Immunol. 165:1771.

Gene ID
16627 View all products for this Gene ID
UniProt
View information about Ly-49AB6 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account