- 145-2C11 (See other available formats)
- Regulatory Status
- Other Names
- CD3ε, T3, CD3
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
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CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Armenian Hamster
- H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32, and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
(PubMed link indicates BioLegend citation)
- Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
- Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
- Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
- Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
- Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
- Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
- Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
- Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
- Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
- Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
- Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
- Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
- Ko SY, et al. 2005. J. Immunol. 175:3309.
- Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
- Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
- Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
- Xiao S, et al. 2007. J. Exp. Med. 204:1691.
- Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
- Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
- Guo Y, et al. 2008. Blood 112:480. PubMed
- Kenna TJ, et al. 2008. Blood 111:2091.
- Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
- Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
- Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
- de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
- Schulteis RD, et al. 2008. Blood 295:G273. PubMed
- Qi Q, et al. 2009. Blood 114:564. PubMed
- Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
- Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
- Yan J, et al. 2014. Vaccine. 32:2833. PubMed
- Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
- Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
- Product Citations
AB_312672 (BioLegend Cat. No. 100307)
AB_312673 (BioLegend Cat. No. 100308)
- Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
Thymocytes (differentiation dependent), mature T cells, NK-T cells
- TCR signal transduction, T cell activation, antigen recognition
- Peptide antigen/MHC-complex
- Cell Type
- NKT cells, T cells, Thymocytes, Tregs
- Biology Area
- Molecular Family
- CD Molecules, TCRs
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.
- Gene ID
- 12501 View all products for this Gene ID
- View information about CD3epsilon on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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