PE anti-mouse CD138 (Syndecan-1) Antibody

Pricing & Availability
Clone
281-2 (See other available formats)
Regulatory Status
RUO
Other Names
Syndecan-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
281-2_PE_1_101211
C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data shown is from gated on myeloid population.
  • 281-2_PE_1_101211
    C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data shown is from gated on myeloid population.
  • 281-2_PE_2_101211
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142503 25 µg £93
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142504 200 µg £241
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Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse mammary gland epithelial cell line NMuMG
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC)
  2. Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF)
  3. Li Q, et al. 2002. Cell 111:635. (IF, IHC)
  4. McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)
Product Citations
  1. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  2. Jash A, et al. 2016. J Immunol. 197: 1159 - 1168. PubMed
  3. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  4. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  5. Pandey SP, et al. 2022. Cell Host Microbe. 30:1003. PubMed
  6. Chen HY, et al. 2022. Elife. 11:. PubMed
  7. Kuang C, et al. 2023. Nat Commun. 14:2093. PubMed
  8. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  9. Wang L, et al. 2019. Cancer Lett. 440-441:135. PubMed
  10. Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed
  11. Clark K, et al. 2015. J Autoimmun. Available online 21 August 2015. PubMed
  12. Wang J, et al. 2021. ACS Nano. 15:14360. PubMed
  13. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  14. Murphy M, et al. 2017. Eur J Immunol. 47:880. PubMed
  15. Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed
  16. Kim Y, et al. 2015. PLoS One. 10:120294. PubMed
  17. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  18. Pan H, et al. 2020. Mol Psychiatry. . PubMed
  19. Tu X, et al. 2022. Nat Commun. 13:6977. PubMed
  20. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
  21. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
  22. Klessing S, et al. 2020. Vaccines (Basel). 8:. PubMed
  23. Utley A, et al. 2020. Cell Rep. 31:107815. PubMed
  24. Liao T, et al. 2017. Front Immunol. 8:1334. PubMed
  25. Chen M, et al. 2016. J Immunol . 194: 2607-15. PubMed
  26. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  27. Roy K, et al. 2019. Immunity. 50:616. PubMed
  28. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  29. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
  30. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  31. Cook KD, et al. 2015. Immunity. 43:703-714. PubMed
  32. Li H, et al. 2020. Nature. 584:274. PubMed
  33. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  34. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  35. Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed
  36. Ribeiro de Almeida C, et al. 2018. Mol Cell. 70:650. PubMed
  37. Cho S, et al. 2018. Nat Commun. 9:2757. PubMed
  38. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  39. Schager AE, et al. 2018. MBio. 9:02379. PubMed
  40. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  41. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  42. Zheng B, et al. 2021. Bioact Mater. 6:3879. PubMed
  43. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  44. Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
  45. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  46. Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed
  47. Che N, et al. 2021. Front Immunol. 12:626310. PubMed
  48. Song W, et al. 2022. Immunity. 55:290. PubMed
  49. Steinmetz TD, et al. 2021. Autophagy. 17:2238. PubMed
  50. BJ L, et al. 2017. J Exp Med . 10.1084/jem.20161461. PubMed
RRID
AB_10915989 (BioLegend Cat. No. 142503)
AB_10916119 (BioLegend Cat. No. 142504)

Antigen Details

Structure
Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
Function
Cell adhesion, cell motility, cell migration, cell proliferation
Cell Type
B cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.

Gene ID
20969 View all products for this Gene ID
UniProt
View information about CD138 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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