PE anti-human TCR Vβ13.2 Antibody

Pricing & Availability
Clone
H132 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor Vβ13.2 chain
Isotype
Mouse IgG1, κ
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Product Citations
publications
H132_PE_061708.jpg
Human peripheral blood lymphocytes stained with CD3 (UCHT1) APC and H132 PE
  • H132_PE_061708.jpg
    Human peripheral blood lymphocytes stained with CD3 (UCHT1) APC and H132 PE
See PE spectral data
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333108 100 tests £150.00
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Description

The Vβ13.2 TCR is a variant of the TCR β chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. Vβ expression in humans and mice has been used to study the effects of superantigens and also to examine the repertoires of T cells involved in inflammatory and autoimmune disease. It is reported that Vβ13.2 TCRs possess very unusually long β-chain CDR3 regions and are expressed on CTL specific for the immunodominant human HLA-B8–restricted HIV-1 nef epitope, FLKEKGGL (FL8). CTL expressing V β13.2 TCRs tolerate naturally arising viral variants in the FL8 epitope that escape recognition by other CTL. The clone H132 recognizes the Vβ13.2 allele product but does not react with the Vβ13.1, 13.3, 13.5 and 13.6 allele products of the Vβ13 subfamily.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TCR Vβ13.2 transfectant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Test format: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2% (w/v) BSA (USA origin).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Choi Y, et al. 1991. Proc. Natl. Acad. Sci. USA 88:8357.
Product Citations
  1. Salio M, et al. 2020. Proc Natl Acad Sci U S A. 117:10465. PubMed
RRID
AB_1027668 (BioLegend Cat. No. 333108)

Antigen Details

Structure
Immunoglobulin superfamily
Distribution
T cells, thymocytes
Function
Antigen recognition, activation of TCR Vβ13.2 bearing T cells
Ligand/Receptor
Peptide bound to MHC
Cell Type
T cells, Thymocytes
Biology Area
Immunology, Adaptive Immunity
Molecular Family
TCRs
Antigen References

1. Choi Y, et al. 1991. Proc. Natl. Acad. Sci. USA 88:8357.
2. Dong T, et al. 2004. J. Exp. Med. 200:1547.

Gene ID
6957 View all products for this Gene ID
UniProt
View information about TCR V beta 13.2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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