PE anti-human CCR10 Antibody

Pricing & Availability
Clone
6588-5 (See other available formats)
Other Names
GPR-2, C-C chemokine receptor 10
Isotype
Armenian Hamster IgG
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Product Citations
publications
A_6588-5_PE_050109.jpg
Human peripheral blood lymphocytes stained with CD3 APC and 6588-5 PE
  • A_6588-5_PE_050109.jpg
    Human peripheral blood lymphocytes stained with CD3 APC and 6588-5 PE
  • B_6588-5_PE_050109.jpg
    Human peripheral blood lymphocytes stained with CLA (HECA-452) FITC and 6588-5 PE (dot plot analysis is derived from CD3+ cell population)
See PE spectral data
Cat # Size Price Quantity Avail. Save
341503 25 tests £84
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341504 100 tests £198
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Description

CCR10, also known as GPR-2, is a G-protein-coupled seven transmembrane CC-chemokine receptor. It is the receptor of CCL27 (CTACK/ALP/ILC/ESkine) and CCL28 (MEC) and is expressed on a small subset of T memory cells, IgA-secreting cells, EBV-immortalized B cells, dermal microvascular endothelial cells and dermal fibroblasts. The interaction of CCR10 with its ligands plays a role in the regulation of T cell homing into cutaneous site and IgA-secreting cells migration.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
N-terminal peptide of human CCR10
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

It has been observed that clone 6588-5 can interact with some tandem-dye antibody conjugates during multi-color staining, potentially leading to unwanted staining. These dyes include PE/Cy7, PE/Cy5, PE/Dazzle™ 594, APC/Cy7, APC/Fire™ 750, and PerCP/Cy5.5.  This interaction can be resolved by sequentially staining with the 6588-5 antibody first and then followed by other antibodies of interest.

Product Citations
  1. Sugita S, et al. 2013. Invest Ophthalmol Vis Sci . 54:6926. PubMed
  2. Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed
  3. Qualai J, et al. 2016. PLoS One. 11: 0156605. PubMed
Publication Library
RRID
AB_1595542 (BioLegend Cat. No. 341503)
AB_1595446 (BioLegend Cat. No. 341504)

Antigen Details

Structure
G-protein-coupled seven transmembrane protein
Distribution

Small subsets of T memory cells and IgA-secreting cells, EBV-immortalized B cells, dermal microvascular endothelial cells and dermal fibroblasts

Function
T cell homing into cutaneous site, regulation of IgA-secreting cells migration
Ligand/Receptor
CCL27 (CTACK/ALP/ILC/ESkine), CCL28 (MEC)
Cell Type
T cells, B cells, Endothelial cells, Fibroblasts
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Hudak S, et al. 2002. J. Immunol. 169:1189
2. Kunkel EJ, et al. 2003. J. Clin. Invest. 111:1001
3. Homey B, et al. 2002. Nature Medicine. 8:157
4. Nakayama T, et al. 2002. J. Virol. 76:3072

Gene ID
2826 View all products for this Gene ID
UniProt
View information about CCR10 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 08/08/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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