PE anti-HDAC1 Phospho (Ser406) Antibody

Pricing & Availability
Clone
BT-15 (See other available formats)
Other Names
Histone Deactylase 1, RPD3L1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
BT-15_PE_HDAC1-phospho_Antibody_1_012219
Untreated HeLa cells (left) and HeLa cells treated with Nocodazole for 24 hours (right), were fixed, permeabilized with True-Phos™ Perm Buffer, and then stained with anti-HDAC1 Phospho (Ser406) Antibody (clone BT-15) PE and DAPI.
  • BT-15_PE_HDAC1-phospho_Antibody_1_012219
    Untreated HeLa cells (left) and HeLa cells treated with Nocodazole for 24 hours (right), were fixed, permeabilized with True-Phos™ Perm Buffer, and then stained with anti-HDAC1 Phospho (Ser406) Antibody (clone BT-15) PE and DAPI.
  • BT-15_PE_HDAC1-phospho_Antibody_2_012219
    Untreated HELA cells (left) and HELA cells treated with Nocodazole for 24 hours (right), were fixed, permeabilized with True-Phos™ Perm Buffer, and then stained with anti-HDAC1 Phospho (Ser406) Antibody (clone BT-15) PE and anti-Histone H3-Phosphorylated (Ser28) Antibody (clone HTA28) Alexa Fluor® 647.
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Cat # Size Price Quantity Avail. Save
611003 25 tests £84
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611004 100 tests £198
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Description

Histone Deacetylase 1 (HDAC1) plays a critical role in various cellular processes, including cell cycle progression, proliferation, and differentiation. The enzyme functions by removing acetyl moieties from histone targets, resulting in histone compaction and alterations in nucleosomal positioning.  Aurora kinases phosphorylate HDAC1 at Ser406 during prophase, immediately after cells begin mitosis, resulting in reduced deacetylase activity of HDAC1. This modification plays an essential role in regulating cell cycle progression, as well as controlling the expression of genes involved in central nervous system development.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Zebrafish2
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human HDAC1 phosphorylated at Serine 406
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

When tested for western blot, this clone produced a band that showed a ~1-3 kD mass shift compared to a pan HDAC1 antibody. This observation is consistent with a previous study of the HDAC1 Phospho (Ser406) site.

This clone recognizes zebrafish HDAC1 phosphorylated at Ser4062.

Application References

(PubMed link indicates BioLegend citation)
  1. Segre CV, et al, 2016. mAbs. 8: 37-42
  2. Loponte S, et al, 2016. Sci Rep, 6: 30213.

Antigen Details

Structure
HDAC1 is a 482 amino acid protein with a predicted molecular weight of 55 kD.
Distribution

Nucleus/Ubiquitously expressed

Function
Phosphorylation of HDAC1 Ser406 results in reduced deactylase activity of the enzyme.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References
  1. Segre CV, et al, 2016. mAbs. 8: 37-42.
  2. Loponte S, et al, 2016. Sci Rep, 6: 30213.
Gene ID
3065 View all products for this Gene ID
UniProt
View information about HDAC1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 01/22/2019

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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