ELISA MAX™ Deluxe Set Human IL-2

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human il-2_122109
  • human il-2_122109
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431804 5 plates £209
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431815 10 Plates £401
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431816 20 Plates £642
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ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!

BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Human IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human IL-2 ELISA MAX™ Capture Antibody (200X)
  • Human IL-2 ELISA MAX™ Detection Antibody (200X)
  • Human IL-2 Standard
  • Avidin-HRP (1000X)
  • Substrate Solution F
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Older lots of these products may contain Substrate Solutions A and B instead of Substrate Solution F mentioned on the TDS and the current manual. Please refer to the lot-specific CoA for the list of the reagents included in the lot and follow the ELISA procedure provided there.

Product Details

Verified Reactivity


As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application Notes

The assay kits will no longer include ELISA microwell plates. Recommended microwell plates can be purchased separately under Cat. No. 423501. Please contact your BioLegend sales representative for bulk pricing quotations.

Application References

(PubMed link indicates BioLegend citation)
  1. Poirier N, et al. 2010. Sci Transl Med. 2:17ra10. (Deluxe kit) PubMed
  2. Redkiewicz P, et al. 2012. Plant Biotechnol J. 10:806. PubMed
  3. Sturner KH, et al. 2014. J Immunol. 193:4439. PubMed
  4. Albring A, et al. 2014. Pharmacol Biochem Behav. 16:126. PubMed
Product Citations
  1. Yang L, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  2. Bröker K, et al. 2019. J Transl Med. 17:6. PubMed
  3. Tello‐Lafoz M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16370-w. PubMed
  4. Zhou J, et al. 2021. Front Immunol. 12:656242. PubMed
  5. Ho CH, et al. 2021. Arthritis Res Ther. 23:199. PubMed
  6. Stürner K, et al. 2014. J Immunol. 193:4439. PubMed
  7. Xu X, et al. 2020. J Cell Biol. 219:00:00. PubMed
  8. Waldrip ZJ, et al. 2021. J Biol Chem. 297:101209. PubMed
  9. Gigliotti CL, et al. 2022. Front Immunol. 13:915963. PubMed
  10. Zhang Y, et al. 2020. Int J Mol Sci. 21:00. PubMed
  11. Karampetsou M, et al. 2016. J Immunol. 196: 4915 - 4924. PubMed
  12. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  13. Albring A, et al. 2014. Pharmacol Biochem Behav. 16:126. PubMed
  14. Redkiewicz P, et al. 2012. Plant Biotechnol J. 10:806. PubMed
  15. Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed
  16. Trinklein ND, et al. 2019. MAbs. 11:639. PubMed
  17. Feng Y, et al. 2022. Life (Basel). 12:. PubMed
  18. Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
  19. Lee C, et al. 2020. Biomaterials. 262:120338. PubMed
  20. Dammermann W, et al. 2017. J Virol Methods. 10.1016/j.jviromet.2017.07.011. PubMed
  21. Lei W, et al. 2022. Blood Cancer J. 12:35. PubMed
4 pg/mL
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2 SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2 HPO4 , 0.2 g KH2 PO4 , 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Sources
T cells
Cell Type
Biology Area
Molecular Family
Gene ID
3558 View all products for this Gene ID
View information about IL-2 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 6    Revision Date: 07/20/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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