ELISA MAX™ Deluxe Set Human IFN-a2

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Other Names
IFNα2, IFNa2, IFNalpha2, IFN alpha 2, Interferon Alpha 2, IFN alpha A, IFNa2a, IFNa2b, IFNa2c
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Human_IFN-alpha2_ELISA_MAX_122818
  • Human_IFN-alpha2_ELISA_MAX_122818
Cat # Size Price Quantity Save
446404 5 plates £155
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Description

Note: This product does not include ELISA microwell plates. Cat. No. 423501 is recommended.

Interferon alpha-2 (IFN-α2) is a cytokine encoded by the IFNA2 gene located on chromosome 9. It belongs to the Type 1 IFN family which includes 13 α subtypes, 1 β, and 1 ω. IFN-α2 was the first of these subtypes to be produced pharmaceutically and is the most widely studied. The mature secreted protein consists of 165 amino acids with 5 α-helices and two disulfide bonds. There are a several allelic variants of IFN-α2, including IFN-α2a, IFN-α2b, and IFN-α2c. IFN-α2 can act on all cell types due to the ubiquitous expression of the Type 1 IFN receptor (IFNAR).

IFN-α2 acts to combat infection by stimulating the production of proteins that inhibit viral replication and prevent viral entry into neighboring cells. IFN-α2 can stimulate the immune system by initiating T and NK cell mediated cytotoxicity. IFN-α2 also can inhibit cancer cell proliferation and activate immune system to target cancer cells. IFN-α2 has been used therapeutically in a variety of viral infections (including hepatitis B and C) and certain cancers (such as melanoma, renal cell carcinoma).


The BioLegend Human IFN-α2 ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The human IFN-α2 capture antibody is first coated on a 96-well plate. Standards and samples are added to the wells and free IFN-α2 binds to the immobilized capture antibody. Next, biotinylated anti-human IFN-α2 detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin horseradish peroxidase is subsequently added, followed by TMB Substrate Solution, producing a blue color in proportion to the concentration of IFN-α2 present in the sample. Finally, the Stop Solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm with a microplate reader. Different IFN-α2 allelic variants can be detected equally.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human IFN-α2 ELISA MAX™ Capture Antibody (200X)
  • Human IFN-α2 ELISA MAX™ Detection Antibody (200X)
  • Human IFN-α2 Standard
  • Matrix Diluent A
  • Avidin-HRP (1000X)
  • Substrate Solution F
  • Coating Buffer B (5X)
  • Assay Diluent A
  • Lot-Specific Certificate of Analysis/ELISA MAX™ Deluxe Set Protocol

Product Details

Reactivity
Human
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
2.94 pg/mL with assay buffer and 2.57 pg/mL with matrix
Standard Range
12.5-800 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper

Antigen Details

Biology Area
Innate Immunity
Molecular Family
Cytokines/Chemokines
Gene ID
3440 View all products for this Gene ID
UniProt
View information about IFN-alpha2 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe sets no longer come with plates, but Coating Buffer and Assay Diluent (for blocking and dilutions) are included in the ELISA MAX™ Deluxe Sets. Plates can be ordered separately (Cat. No. 423501), and instructions on coating the plates are in the sets’ product manuals.

Go To Top Version: 2    Revision Date: 04/02/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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