ELISA MAX™ Deluxe Set Human CCL8 (MCP-2)

Pricing & Availability
Regulatory Status
RUO
Other Names
MCP2, Chemokine (C-C motif) ligand 8, small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2)
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Product Citations
publications
CCL8_MCP-2_Human_ELISA_Kit_Deluxe_122915
  • CCL8_MCP-2_Human_ELISA_Kit_Deluxe_122915
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442204 5 plates £238
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Description

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Chemokine (C-C motif) ligand 8 (CCL8), also known as monocyte chemoattractant protein 2 (MCP-2), is a protein which in humans is encoded by the CCL8 gene. CCL8 belongs to the CC chemokine family and is up-regulated in inflammed tissues. It binds to several G proteincoupled receptors including CCR1, CCR2, CCR3 and CCR5. It activates and attracts many different immune cells. High levels of CCL8 are associated with tuberculosis (TB) and graft-versus-host disease (GVHD) following hematopoietic stem-cell transplantation (HSCT). Since it recognizes the receptor CCR5, which is a major co-receptor for HIV-1, CCL8 has been investigated for its potential as an inhibitor for HIV infection.

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience, and performance of a high-end, commmercial ELISA kit for a fraction of the price!

BioLegend's ELISA Max™ Sets, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing cost effectiveness, value, and ease of use.

The ELISA Max™ Set Deluxe is specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumathoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human CCL8 ELISA MAX™ Capture Antibody (200X)
  • Human CCL8 ELISA MAX™ Detection Antibody (200X)
  • Human CCL8 Standard
  • Avidin-HRP (1000X)
  • Substrate Solution D
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)
  • Assay Buffer D

Product Details

Verified Reactivity
Human
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Product Citations
  1. Eitas T, et al. 2017. PLoS One. 10.1371/journal.pone.0184164. PubMed
Sensitivity
4.0 pg/mL
Standard Range
7.8 - 500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Soaline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCL, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20. BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (acid solution e.g. 2N H2SO4) BioLegend Cat. No. 423001
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent Paper

Antigen Details

Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
6355 View all products for this Gene ID
UniProt
View information about CCL8 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 3    Revision Date: 09/12/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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