HRP anti-His Tag Antibody

Pricing & Availability
Clone
J099B12 (See other available formats)
Regulatory Status
RUO
Other Names
Histidine Tag
Isotype
Mouse IgG1, κ
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Product Citations
publications
J099B12_His_Tag_Antibody_ELISA_101614
A 96 well plate is coated with 100 µL of a His-tagged recombinant protein at 100 ng/mL. The coated protein is detected with HRP-conjugated anti-His tag antibody at concentration ranged from 1 to 0.008 µg/mL.
  • J099B12_His_Tag_Antibody_ELISA_101614
    A 96 well plate is coated with 100 µL of a His-tagged recombinant protein at 100 ng/mL. The coated protein is detected with HRP-conjugated anti-His tag antibody at concentration ranged from 1 to 0.008 µg/mL.
  • J099B12_His_Tag_Antibody_WB_101614
    A His-tagged recombinant protein is diluted in cell-culture medium. The diluted protein is loaded to 12% SDS PAGE gel in amount of 40, 20, and 10 ng. After electrophoresis, the His-tagged recombinant protein is detected with 0.3 µg/mL of the HRP-conjugated anti-His tag monoclonal antibody.
Cat # Size Price Quantity Avail. Save
652503 25 µg £65.00
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652504 100 µg £239.00
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Description

This antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. It can be used for Western blotting to detect linear His repeat (His6 or more).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
All species (6x His tag epitope)
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated His-tag peptide
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solution with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light. Avoid exposure to sodium azide.
Application

ELISA - Quality tested
WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by direct ELISA assay. For direct ELISA, a concentration range of 0.01 - 1.0 µg per ml is recommended. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. Avoid using diluent solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the end-user titrate each lot of reagents for optimal performance.

Application References

(PubMed link indicates BioLegend citation)
  1. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
  2. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
Product Citations
  1. Hay CE, et al. 2018. PLoS One. 13:29. PubMed
  2. Zhang D, et al. 2020. Oncoimmunology. 9:1744921. PubMed
  3. Zhao Y, et al. 2019. Cancer Biol Ther. 0.925. PubMed
  4. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
RRID
AB_2734520 (BioLegend Cat. No. 652503)
AB_2563555 (BioLegend Cat. No. 652504)

Antigen Details

Function
Recombinant poly-histidine tag detection
Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about His Tag on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/01/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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