HRP anti-BACE1 Antibody

Pricing & Availability
Clone
A17035K (See other available formats)
Other Names
Beta-Secretase 1, Membrane-Associated Aspartic Protease 2, Beta-Site APP Cleaving Enzyme 1, Aspartyl Protease 2, Memapsin-2, Asp 2, BACE, ASP2, Beta-Site Amyloid Beta A4 Precursor Protein-Cleaving Enzyme, Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1, Transmembrane Aspartic Proteinase Asp2, Beta-Secretase 1 Precursor Variant 1, Beta-Site APP-Cleaving Enzyme 1, APP Beta-Secretase
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17035K_HRP_BACE1_Antibody_1_110718
Western blot of HRP anti-BACE1 antibody (clone A17035K). Lane 1: Molecular weight marker; Lane 2: 20 µg of human cerebellum membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody at 4°C overnight. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A17035K_HRP_BACE1_Antibody_1_110718
    Western blot of HRP anti-BACE1 antibody (clone A17035K). Lane 1: Molecular weight marker; Lane 2: 20 µg of human cerebellum membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody at 4°C overnight. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
Cat # Size Price Quantity Avail. Save
852903 25 µg £84
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852904 100 µg £178
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Description

Beta-site APP-cleaving enzyme 1 (BACE1), also known as aspartyl protease 2 (ASP-2) and memapsin 2, is a type I transmembrane aspartic protease with 501 amino acid that is related to the pepsin family and the retroviral aspartic proteases. The BACE1 catalytic domain contains aspartic protease active site motifs at amino acids 93-96 and 289-292, with each motif containing the highly conserved sequence defining aspartic proteases, D(T/S)G(T/S). BACE1 is expressed at low levels in most cell types of the body, although it is more highly expressed in neurons. In the brain, the protein is highly expressed in the substantia nigra, locus coruleus, and medulla oblongata. It is localized within acidic intracellular compartments including endosomes and trans-Golgi network (TGN). In the brain, BACE1 is involved in proteolytic processing of the amyloid precursor protein (APP) which generates the b amyloid peptide- a peptide that is associated with Alzheimer’s disease. BACE1 cleaves APP at the N-terminus of the b amyloid peptide sequence, between residues 671 and 672, which leads to extracellular release of b-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by g-secretase. The physiological function of BACE1 is not clearly understood, although it may modulate sodium currents by cleavage of voltage-gated Na+ channel accessory subunits (Navβ1-4). Also, BACE1 has been implicated in the regulation of myelination and myelin sheath thickness and in the control of motor coordination via cleavage of neuregulin-1 and neuregulin-3, and also in axon guidance via cleavage of close homolog of L1 (CHL1).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human BACE1 protein.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783427 (BioLegend Cat. No. 852903)
AB_2783428 (BioLegend Cat. No. 852904)

Antigen Details

Structure
Human BACE1 is a 501 amino acid protein with a molecular mass of 56 kD and exists as 4 isoforms.
Distribution

Tissue sources: Ubiquitously expressed. Highest levels in the brain and pancreas.
Cellular distribution: Plasma membrane, endoplasmic reticulum, and golgi apparatus.

Function
BACE1 is involved in proteolytic processing of the amyloid precursor protein (APP). It cleaves APP at the N-terminus of the amyloid β peptide sequence, between residues 671 and 672 of APP.
Interaction
APP, IL1-R2, PSGL-1, ST6Gal-1, GGA1, GGA2, GGA3, RTN3, RTN4, SNX6, PCSK9, NAT8 and NAT8B.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Enzymes and Regulators, Secretases
Antigen References
  1. Vassar R, et al. 2014. J. Neurochem. 130:4-28.
  2. Kandalepas PC, Vassar R. 2014. Curr. Alzheimers Res. 11:441-9.
  3. Kandalepas PC, Vassar R. 2012. J. Neurochem. 120(Suppl. 1):55-61.
  4. Gruninger-Leitch F, et al. 2002. J. Biol. Chem. 277:4687-93.
  5. Koelsch G. 2017. Molecules. 22(10): E1723.
Gene ID
23621 View all products for this Gene ID
UniProt
View information about BACE1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/12/2018

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