- A16002B (See other available formats)
- Regulatory Status
- Other Names
- Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, HIES
- Mouse IgG1, κ
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- Product Citations
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Tyrosine phosphorylation of STAT3 at Tyr705 occurs in response to LIF, IL-6, leptin, OSM, EGF, PDGF, and HGF. It plays a key role in cell growth and apoptosis through mediating expression of a variety of genes in response to the stimuli.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Human STAT3 peptide phosphorylated at Y705. Complete Freund's adjuvant.
- Phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide
- The Go-ChIP-Grade™ Purified antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ChIP - Quality tested
WB, ICFC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by ChIP. For ChIP, the suggested use of this reagent is 1:50 dilution by volume. For Western blotting, the suggested use of this reagent is 0.05 - 0.5 µg per ml. For intracellular flow cytometry using our True-Phos™ Perm Buffer in Whole Blood Protocol, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Recognition of pan STAT3, weak cross-reactivity with mouse (C57BL/6) STAT3 Phospho.
The STAT3 Phospho (Tyr705) antibody recognizes the regulatory tyrosine phosphorylation of human STAT3 protein and has been shown to be useful for Western blotting.
This clone recognizes both unphosphorylated and phosphorylated (Tyr705) STAT3 protein.
AB_2716053 (BioLegend Cat. No. 690405)
AB_2716054 (BioLegend Cat. No. 690406)
- STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
- STAT3 is tyrosine phosphorylated by receptor kinases in response to a variety of cytokines and growth factors. It forms homo- or heterodimer with STAT1 when tyrosine is phosphorylated, and then translocates to nucleus, acting as a transcription regulator. It is also essential for the differentiation of TH17 cells, which is involved in autoimmune diseases. The small GTPase Rac1 binds and regulates activity of STAT3.
- When activated, STAT3 dimerizes and translocates to the nucleus where it regulates gene expression.
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells
- Molecular Family
- Nuclear Markers, Phospho-Proteins
- Antigen References
1. Akira S, et al. 1994. Cell. 77:63.
2. Zhang X, et al. 1995. Science 267:1990.
3. Sanchez-Margalet V, et al. 2001. Cell. Immunol. 211:30.
4. Simon A, et al. 2000. Science 290:144.
5. Hoey T, et al. 1999. Adv. Immunol. 71:145.
- Gene ID
- 6774 View all products for this Gene ID
- View information about STAT3 Phospho on UniProt.org
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