FITC anti-mouse CD172a (SIRPα) Antibody

Pricing & Availability
P84 (See other available formats)
Regulatory Status
Other Names
SHPS-1, BIT, P84, PTPNS1, CD172 antigen-like family member A
Rat IgG1, κ
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Product Citations
C57BL/6 mouse bone marrow cells were stained with CD11b APC and CD172a (clone P84) FITC (top) or rat IgG1, κ FITC isotype control (bottom). Data shown was gated on the myeloid cell population.
  • P84_FITC_1_051012
    C57BL/6 mouse bone marrow cells were stained with CD11b APC and CD172a (clone P84) FITC (top) or rat IgG1, κ FITC isotype control (bottom). Data shown was gated on the myeloid cell population.
  • P84_FITC_2_051012
  • P84_FITC_3_050319
    Fixed whole mount mouse spleen was stained with FITC CD172a (red) (clone P84), Alexa Fluor® 647 CD11c (yellow) (clone N418), and PE CD11b (blue) (clone M1/70). Isotype controls at the same concentrations were used for the negative control. Cells were mounted in Prolong Gold and imaged with a Leica SP8 confocal. Image courtesy of Grzegorz Chodaczek and Zbigniew Mikulski at LIAI.
Compare all formats See FITC spectral data
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144005 25 µg £70
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144006 100 µg £139
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CD172a, also known as SIRPα, is a type I transmembrane protein with one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, and two ITIM motifs and a proline-rich region in the cytoplasmic tail. CD172a is expressed by monocytes, macrophages, myeloid cells, and neuronal tissue. The phosphorylation of SIRPα ITIMs induces the recruitment and activation of the tyrosine phosphatases PTPN6 and PTPN11, resulting in the negative regulation of several biological processes. The ligands of CD172a are CD47, SP-A, and SP-D.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Mouse brain membrane protein
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking SIRPa interaction with CD474, in vivo blocking of dendritic cell migration3, enhancing of macrophage phagocytosis2,4, immunohistochemical staining of cerebellum frozen sections1, immunoprecipitation2,4, and spatial biology (IBEX)5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Comu S, et al. 1997. J. Neurosci. 17:8702. (IHC)
  2. Gresham HD, et al. 2000. J. Exp. Med. 191:515. (IP)
  3. Fukunaga A, et al. 2004. J. Immunol. 172:4091. (Block)
  4. Oldenborg PA, et al. 2000. Science 288:2051. (Block, IP)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Gotoh K et al. 2018. Cell reports. 25(7):1800-1815 . PubMed
  2. Ding X, et al. 2021. Nat Commun. 12:2030. PubMed
  3. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  4. Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
  5. Marangoni F, et al. 2021. Cell. . PubMed
  6. Perry JSA, et al. 2018. Immunity. 48:923. PubMed
  7. Fujita K, et al. 2019. Proc Natl Acad Sci U S A. 116:14714. PubMed
  8. Gozgit JM, et al. 2021. Cancer Cell. :. PubMed
  9. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  10. Freed-Pastor WA, et al. 2021. Cancer Cell. 39:1342. PubMed
AB_11204432 (BioLegend Cat. No. 144005)
AB_11204425 (BioLegend Cat. No. 144006)

Antigen Details

Type I transmembrane protein

Monocytes, macrophages, myeloid cells, neuronal tissue

Negative regulation of several biological processes
CD47, SP-A, SP-D
Cell Type
Dendritic cells, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
Antigen References

1. Zhao XW, et al. 2011. P. Natl. Acad. Sci. USA 108:18342.
2. Verjan-Garcia N, et al. 2011. J. Immunol. 187:2268.
3. Sato-Hashimoto M, et al. 2011. J. Immunol. 187:291.
4. Raymond M, et al. 2010. Eur. J. Immunol. 40:3510.

Gene ID
19261 View all products for this Gene ID
View information about CD172a on

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Go To Top Version: 3    Revision Date: 10/25/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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