FITC anti-mouse CD154 (CD40L) Antibody

Pricing & Availability
Clone
SA047C3 (See other available formats)
Other Names
CD40L, gp39, TRAP, T-BAM, TNFSF5, CD40 Ligand
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
SA047C3_FITC_CD154_Antibody_091019.png
Enriched BALB/c mouse splenic T cells were stimulated with PMA plus ionomycin for 6 hours and then stained with mouse CD154 (CD40L) (clone SA047C3) FITC (flled histogram) or rat IgG2a FITC isotype control (open histogram).
  • SA047C3_FITC_CD154_Antibody_091019.png
    Enriched BALB/c mouse splenic T cells were stimulated with PMA plus ionomycin for 6 hours and then stained with mouse CD154 (CD40L) (clone SA047C3) FITC (flled histogram) or rat IgG2a FITC isotype control (open histogram).
See FITC spectral data
Cat # Size Price Quantity Avail. Save
157005 25 µg £44
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157006 100 µg £158
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Description

CD154 has been kown a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. CD154 is an accessory molecule expressed predominantly on activated CD4+ lymphocytes that bind CD40. CD154 plays an important role in T-B cell costimulation and Ig class switching.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mCD40L-transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
RRID
AB_2814085 (BioLegend Cat. No. 157005)
AB_2814086 (BioLegend Cat. No. 157006)

Antigen Details

Structure
A 39 kD TNF superfamily memeber
Distribution

Surface activated CD4+ T cells,B cells, macrophages, dendritic cells, mast cells, NK cells, polymorphs, monocytes, and activated platelets.

Function
Survival signaling to CD40+ B cells and Ig class switching.
Ligand/Receptor
CD40
Cell Type
B cells, Basophils, Dendritic cells, Fibroblasts, Macrophages, Mast cells, NK cells, Platelets, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Gene ID
959 View all products for this Gene ID
UniProt
View information about CD154 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/10/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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