FITC anti-human SSEA-5 Antibody

Pricing & Availability
Clone
8e11 (See other available formats)
Regulatory Status
RUO
Other Names
Stage-Specific Embryonic Antigen-5
Isotype
Mouse IgG1, κ
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Product Citations
publications
8E11_FITC_SSEA-5_Antibody_FC_020813
Human teratocarcinoma cell line NCCIT, was stained with SSEA-5 (clone 8e11) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
  • 8E11_FITC_SSEA-5_Antibody_FC_020813
    Human teratocarcinoma cell line NCCIT, was stained with SSEA-5 (clone 8e11) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
Compare all formats See FITC spectral data
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355208 100 tests £238
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Description

Stage-Specific Embryonic Antigen-5 (SSEA-5) is an oligosaccharide that contains the motif Fuc1-2Galβ1-3GlcNAcβ, which is characteristic of H-1 antigens. SSEA-5 is expressed on undifferentiated embryonic stem cells and pluripotent stem cells. It is developmentally regulated, and its expression is lost with cell differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human embryonic stem cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1 and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Tang C, et al. 2011. Nat Biotechnol. 29:829. (IHC, IP)
RRID
AB_2561827 (BioLegend Cat. No. 355208)

Antigen Details

Structure
Glycan, H type-1 backbone, 127, and >190 kD
Distribution

Embryonic stem cells, pluripotent stem cells

Cell Type
Embryonic Stem Cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Andrews PW. 2011. Nat. Biotechnol. 29:803.
2. Itskovitz-Eldor J. 2011. Cell. Stem. Cell. 9:291.

Gene ID
NA
UniProt
View information about SSEA-5 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/13/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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