FITC anti-human/mouse Granzyme B Antibody

Pricing & Availability
Clone
GB11 (See other available formats)
Regulatory Status
RUO
Other Names
Granzyme-2, serine protease B, CCP1, Asp-ase, CTLA-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_GB11_FITC_082409
Human peripheral blood lymphocytes surface stained with CD8 PE, then intracellular stained with GB11 FITC
  • A_GB11_FITC_082409
    Human peripheral blood lymphocytes surface stained with CD8 PE, then intracellular stained with GB11 FITC
  • B_GB11_FITC_082409
    Human peripheral blood lymphocytes surface stained with CD8 PE, then intracellular stained with mouse IgG1 FITC isotype control
See FITC spectral data
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515403 25 tests £82.00
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Description

Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Cross-Reactivity: Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Wever PC, et al. 1998. Immunology. 93:383
  2. Arens R, et al. 2004. J. Exp. Med. 199:1595
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  6. Nacer A, et al. 2014. PLoS Pathog. 10:1004528. PubMed
  7. Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed
Product Citations
  1. Jeng MY, et al. 2018. J Exp Med. 215:51. PubMed
  2. Zhang J, et al. 2018. Nature. 553:91. PubMed
  3. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
  4. Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed
  5. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  6. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
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  9. Sagebiel AF, et al. 2019. Nat Commun. 10:975. PubMed
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  11. Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
  12. Shen T, et al. 2019. Cell Death Discov. 5:123. PubMed
  13. Lettau M, et al. 2018. Int Immunol. 30:215. PubMed
  14. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  15. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  16. van Montfoort N, et al. 2018. Cell. 175:1744. PubMed
  17. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  18. Chan LC, et al. 2019. J Clin Invest. 129:3324. PubMed
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  20. Di Blasi D, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.510416667. PubMed
  21. Ron–Harel N, et al. 2019. Cell Rep. 28:3011. PubMed
  22. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
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  24. Kansy BA, et al. 2017. Cancer Res. 77:6353. PubMed
  25. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  26. Zhang C, et al. 2020. Cell Metabolism. 31(1):148-161.e5.. PubMed
  27. Walk J, et al. 2020. Front Immunol. 2.405555556. PubMed
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  32. Rafiq S, et al. 2018. Nat Biotechnol. 36:847. PubMed
  33. Kmieciak M, et al. 2011. J Immunol. 187:708. PubMed
  34. Wiede F, et al. 2014. J Autoimmun. 53:105. PubMed
  35. Baker G, et al. 2014. Cancer Res. 74:5079. PubMed
  36. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
  37. Fujigaki J, et al. 2015. PLoS One. 10: 0132521. PubMed
  38. Leeansyah E, et al. 2015. PLoS Pathog. 11: 1005072. PubMed
  39. Durand J, et al. 2015. J Immunol. 195: 5035 - 5044. PubMed
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  47. Dubrot J, et al. 2021. Immunity. 54(3):571-585.e6. PubMed
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RRID
AB_2114575 (BioLegend Cat. No. 515403)

Antigen Details

Structure
32 kD serine protease
Distribution
Cytotoxic T-cells and NK cells, low on granulocytes, B cells and activated dendritic cells
Function
Induction of cell death and apoptosis
Ligand/Receptor
Mannose-6-phosphate receptor
Cell Type
T cells, NK cells, B cells, Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Proteases, Enzymes and Regulators
Antigen References

1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203
2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885
3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610
4. Wagner C, et al. 2008. Mol. Immunol. 45:1761

Gene ID
3002 View all products for this Gene ID 14939 View all products for this Gene ID 171528 View all products for this Gene ID
UniProt
View information about granzyme b on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 05/31/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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