ELISA MAX™ Deluxe Set Human Total Tau

Pricing & Availability
Regulatory Status
RUO
Other Names
Microtubule-Associated Protein Tau, Phf-Tau, Paired Helical Filament-Tau, Neurofibrillary Tangle Protein, Microtubule-Associated Protein Tau, Isoform 4, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1
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ELISA-MAX_Deluxe_Human_Total-Tau_111422
  • ELISA-MAX_Deluxe_Human_Total-Tau_111422
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449204 5 plates £256
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Description

Tau proteins constitute a family of six isoforms expressed by the MAPT (Microtubule-Associated Proteins/Tau) gene. Full-length natural tau protein consists of 441 amino acids (Tau-441, 2N4R). Tau isoforms are named based on the number of amino-terminal inserts (0N, 1N, and 2N) or carboxyl-terminal microtubule binding domain repeats (3R and 4R). Tau proteins are expressed mainly in the neurons of the central nervous system (CNS) in human brain. They help stabilize the internal skeleton of neurons in the brain. This internal skeleton has a tube-like shape through which nutrients and other essential substances travel to reach different parts of the neuron. Tau proteins are important biomarkers for neurodegenerative disease research such as Alzheimer’s disease (AD) and Parkinson disease (PD). Biolegend’s ELISA MAX™ Deluxe Set Human Total Tau kit can detect all six Tau isoforms: 2N4R, 1N4R, 0N4R, 2N3R, 1N3R, 0N3R.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human Total Tau Capture Antibody (200X)
  • Human Total Tau Detection Antibody (200X)
  • Human Total Tau Standard
  • Streptavidin-Polymer HRP (1000X)
  • Substrate Solution D
  • Coating Buffer B (5X)
  • Assay Diluent B (5X)
  • Blocking Buffer A (2X)

Product Details

Verified Reactivity
Human
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
0.68 pg/mL
Standard Range
7.9 - 500 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Type
Neurons
Biology Area
Immunology, Neurodegeneration, Neuroscience
Molecular Family
Microtubules, Tau
Gene ID
NA
UniProt
View information about Tau on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 11/14/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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