ELISA MAX™ Deluxe Set Human Properdin

Pricing & Availability
Regulatory Status
RUO
Other Names
CFP, Complement Factor P
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ELISA-MAX_Deluxe_Human_Properdin
  • ELISA-MAX_Deluxe_Human_Properdin
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448904 5 plates £238
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Description

Properdin, known as CFP or complement factor P, is a protein that activates the complement regulatory proteins of the innate immune system. It is a glycoprotein produced by leukocytes and found in plasma. It positively regulates alternative pathway activity by stabilizing C3 and C5 convertases. Properdin’s involvement in complement activity changes the cellular microenvironment, therefore contributing to both the innate and adaptive immune response. This includes production of inflammatory cytokines, immune cell infiltration, antigen presenting cell maturation and tissue damage. As an inflammatory modulator, it can enhance damaging inflammation. Studies have shown its implication in several renal diseases, highlighting its role as a potential urinary biomarker. Its role in inflammatory conditions is multifaceted and as such, there is potential for the development of new disease therapies.

Biolegend’s ELISA MAX™ Deluxe Set Human Properdin ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA), utilizing a monoclonal capture antibody and biotinylated monoclonal antibody specific for human Properdin. This set contains necessary reagents for the accurate quantification of natural and recombinant human Properdin in serum, plasma, urine and other biological fluids. Biolegend’s ELISA MAX™ Deluxe Sets are cost-effective, sensitive, accurate, and robust.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human Properdin ELISA MAX™ Capture Antibody (200X)
  • Human Properdin ELISA MAX™ Detection Antibody (200X)
  • Human Properdin Standard
  • Avidin-HRP (1,000X)
  • Substrate Solution D
  • Coating Buffer B (5X)
  • Assay Diluent B (5X)

Product Details

Verified Reactivity
Human
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
7.21 pg/mL
Standard Range
23.44 - 1500 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Molecular Family
Scaffold Proteins
Gene ID
NA
UniProt
View information about Properdin on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 03/17/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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