Direct-Blot™ HRP anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody

Pricing & Availability
Clone
4B11B69 (See other available formats)
Other Names
P44/42 MAP (motogen activated protein) Kinase, Extracelluar signal-regulated kinase (ERK)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
4B11B69_ERK1slash2Phospho_WB_Image_062116
15 µg of total protein extract from Jurkat cells treated with PMA was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-ERK1/2 Phospho (Thr202/Tyr204) (clone 4B11B69). Proteins were visualized using chemiluminescence detection.
  • 4B11B69_ERK1slash2Phospho_WB_Image_062116
    15 µg of total protein extract from Jurkat cells treated with PMA was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-ERK1/2 Phospho (Thr202/Tyr204) (clone 4B11B69). Proteins were visualized using chemiluminescence detection.
Cat # Size Price Quantity Avail. Save
675506 25 µl £57
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675505 100 µl £195
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Description

ERK1/2 are members of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide (TGFLT*EY*VATRC) conjugated to KLH.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

RRID
AB_2566700 (BioLegend Cat. No. 675505)

Antigen Details

Structure
ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology.
Distribution

Cytoplasm. Nucleus.

Function
ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1/2 are phosphorylated at Thr202 and Tyr204 and in turn transduce signals to downstream substrates.
Interaction
ERK1/2 phosphorylates a wide range of downstream substrates, such as ATF2, BCL6, EIF4EBP1, CANX, MAP2, DAPK1, RAF1, SYK, and GRB10.
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Phospho-Proteins, Postsynaptic proteins, Protein Kinases/Phosphatase
Antigen References

1. Futran AS, et al. 2013. Curr. Biol. 23:R972.
2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320.
3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299.
4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320.
5. Torii S, et al. 2004. J. Biochem. 136:557.
6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814.

Gene ID
5594 View all products for this Gene ID
UniProt
View information about ERK1/2 Phospho on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 06/21/2016

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