Brilliant Violet 650™ anti-mouse CD155 (PVR) Antibody

Pricing & Availability
Clone
TX56 (See other available formats)
Other Names
PVR (poliovirus receptor) homolog
Isotype
Rat IgG2a, κ
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Product Citations
publications
TX56_BV650_CD155_Antibody_091217
C57BL/6 thymocytes were stained with anti-mouse CD4 (clone GK1.5) PE, anti-mouse CD8a (clone 53-6.7) APC, and either anti-mouse CD155 (clone TX56) Brilliant Violet 650™ (filled histogram) or rat IgG2a, κ (clone RTK2758) Brilliant Violet 650™ (dashed histogram). Histograms shown are derived from the following populations: CD4-8+ (top left), CD4+CD8+ (top right), CD4+CD8- (bottom right), CD4-CD8- (bottom left).
  • TX56_BV650_CD155_Antibody_091217
    C57BL/6 thymocytes were stained with anti-mouse CD4 (clone GK1.5) PE, anti-mouse CD8a (clone 53-6.7) APC, and either anti-mouse CD155 (clone TX56) Brilliant Violet 650™ (filled histogram) or rat IgG2a, κ (clone RTK2758) Brilliant Violet 650™ (dashed histogram). Histograms shown are derived from the following populations: CD4-8+ (top left), CD4+CD8+ (top right), CD4+CD8- (bottom right), CD4-CD8- (bottom left).
See Brilliant Violet 650™ spectral data
Cat # Size Price Quantity Avail. Save
131521 50 µg £188
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Description

Mouse CD155, also known as homolog of polivirus receptor (PVR), is the founding member of a subfamily of immunoglobulin-like adhesion receptors (nectins). Mouse CD155 is expressed on a wide variety of cells of hematopoietic origin apart from its existence on the epithelial cells. The engagement of CD155 with CD226 and CD96 induces cytotoxicity of NK cells and CTL.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CD155 transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Tahara-Hanaoka S, et al. 2004. Int. Immunol. 16:533.
  2. Maier MK, et al. 2007. Eur. J. Immunol.. 37:2214.
  3. Bottino C, et al. 2003. J. Exp. Med. 198:557.
  4. Dressel R, et al. 2010. FASEB J. 9:134. PubMed
RRID
AB_2716161 (BioLegend Cat. No. 131521)

Antigen Details

Structure
70 kd immunoglobulin-like molecule with three Ig-like domains.
Distribution

CD155 is expressed at cell junctions on the primary vascular endothelial cells. Moreover, mouse CD155 is highly expressed on DP thymocytes and expression level is dropped in peripheral single positive CD4+ and CD8+ T cells. CD155 can be detected on T reg, other activated T cells and on NKT cells. Resident monocytes express moderate amount of CD155, but upregulated its expression during inflammation. Other cells, such as IEL, neutrophils and subsets of B cells were found to express CD155 to varying extents.

Function
Apart from its function of adhering junction among contacting epithelial cells, the interaction of DNAM-1 (CD226) with its ligands CD155 and CD112 (nectin 2) induces cytotoxcity of NK cells and CD8+ T cells and cytokine secretion.
Ligand/Receptor
CD226 (DNAM-1), CD96
Cell Type
B cells, Endothelial cells, Neutrophils, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Mendelsohn C, et al. 1989. Cell 56:855.

Gene ID
52118 View all products for this Gene ID
UniProt
View information about CD155 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 09/13/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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