Brilliant Violet 650™ anti-human CD3 Antibody

Pricing & Availability
Clone
OKT3 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
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Product Citations
publications
OKT3_BV650_020312
Human peripheral lymphocytes were stained with CD3 (clone OKT3) Brilliant Violet 650™.
  • OKT3_BV650_020312
    Human peripheral lymphocytes were stained with CD3 (clone OKT3) Brilliant Violet 650™.
See Brilliant Violet 650™ spectral data
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317323 25 tests £133.00
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317324 100 tests £260.00
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Description

CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.

Clone OKT3 can block the binding of clones SK7 and UCHT1.4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Li B, et al. 2005. Immunology 116:487.
  5. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
  6. Alter G, et al. 2008. J. Virol. 82:9668. PubMed
  7. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
  8. Pinto JP, et al. 2010. Immunology. 130:217. PubMed
  9. Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed
Product Citations
  1. Robert R, et al. 2017. PLoS One. 10.1371/journal.pone.0184278. PubMed
  2. Garfall AL, et al. 2018. JCI Insight. 3. PubMed
  3. Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
  4. Bradley T et al. 2018. Cell. 175(2):387-399 . PubMed
  5. Savage TM, et al. 2018. JCI Insight. 3:. PubMed
  6. Querfeld C, et al. 2018. Cancer Immunol Res. 6:900. PubMed
  7. Franco LM, et al. 2019. J Exp Med. 216:384. PubMed
  8. Escudero–Pérez B, et al. 2019. JCI Insight. 4:e126070. PubMed
  9. Mikulak J, et al. 2019. JCI Insight. 4:e125884. PubMed
  10. Booth JS, et al. 2019. Hum Vaccin Immunother. :1. PubMed
  11. Tilton C, et al. 2013. J Virol Methods. 166:376. PubMed
  12. Boudreau J, et al. 2014. PLoS One. 9:99543. PubMed
  13. Riou C, et al. 2014. PLoS One. 9:102178. PubMed
  14. Lucera M, et al. 2014. J Virol. 88:10803. PubMed
  15. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  16. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  17. Boyle M, et al. 2015. J Infect Dis. 212: 416-425. PubMed
  18. Boyle M, et al. 2015. PLoS Pathog. 11: 1005041. PubMed
  19. Xu Y, et al. 2015. J Vis Exp. 99: 52863. PubMed
  20. Mattiola I, et al. 2015. J Immunol. 195: 2818-2828. PubMed
  21. Rodda LB, et al. 2020. Cell. 184(1):169-183.e17. PubMed
  22. Pierce CA, et al. 2020. Sci Transl Med. 12:00. PubMed
  23. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  24. Clayton KL, et al. 2021. Cell Host Microbe. 29(3):435-447.e9. PubMed
  25. Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed
RRID
AB_11126748 (BioLegend Cat. No. 317323)
AB_2563352 (BioLegend Cat. No. 317324)

Antigen Details

Structure
Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) form the CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
  2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
  3. Lanier L, et al. 1986. J. Immunol. 137:2501.
Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/12/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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