Biotinylated Recombinant Mouse B7-2/CD86-Fc Chimera (carrier-free)

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Other Names
CD86, B7-2, T lymphocyte activation antigen CD86, Activation B7-2 antigen, CD28 antigen ligand 2, CD28LG2, B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1
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Mouse_B7-2_CD86_Chimera-Biotinylated_CF_RECOM_1_021319
When mouse CTLA-4 (Cat. No. 591802) is immobilized at 1.0 µg/mL, Mouse B7-2/CD86-Fc Chimera Biotinylated Protein binds with EC50 of 6 - 24 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Mouse_B7-2_CD86_Chimera-Biotinylated_CF_RECOM_1_021319
    When mouse CTLA-4 (Cat. No. 591802) is immobilized at 1.0 µg/mL, Mouse B7-2/CD86-Fc Chimera Biotinylated Protein binds with EC50 of 6 - 24 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Mouse_B7-2_CD86_Chimera-Biotinylated_CF_RECOM_2_021319
    Stability testing for Mouse B7-2/CD86-Fc Chimera Biotinylated Protein. Mouse B7-2/CD86-Fc Chimera Biotinylated Protein was aliquoted in PBS, pH 7.2, 5% glycerol at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested for their ability to bind immobilized mouse CTLA-4 (Cat. No. 591802).
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780502 10 µg £84
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780504 25 µg £165
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780506 100 µg £491
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Description

CD86, also known as B7-2, is a member of the cell surface immunoglobulin superfamily. B7 family members are transmembrane molecules that exist predominantly as monomers. They are classified into three groups according to their functions. Group I members include B7-1, B7-2, and B7-H2. These members are either co-stimulatory or co-inhibitory in modulating T cell response. Group II members include B7-H1 (PD-L1) and B7-DC (PD-L2) which are ligands for PD-1. These members are involved in regulating induction and maintenance of peripheral immune tolerance. Group III members include B7-H3 and B7-H4 whose role in T cell regulation has yet to be defined. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Source
Mouse B7-2/CD86, amino acids Val26-Glu245 (Accession # P42082) with a C-terminal human IgG1 Fc-6His tag was expressed in 293E cells.
Molecular Mass
The 486 amino acid recombinant protein has a predicted molecular mass of approximately 54.9 kD. The DTT-reduced protein migrates at approximately 70 kD and and non-reduced protein migrates at approximately 140 kD by SDS-PAGE. The predicted N-terminal amino acid is Val.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 μm filtered protein solution is in PBS, pH 7.2, 5% Glycerol.
Endotoxin Level
Less than 0.1 EU per μg protein as determined by the LAL method.
Concentration
10-25 µg sizes are bottled at 200 µg/mL. 100 µg size is lot-specific and bottled at the concentration indicated on the vial. (Please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
When mouse CTLA4a-Fc (Cat. No. 591802) is immobilized at 1.0 μg/mL (100 μL/ well), biotinylated mouse B7-2/CD86-Fc Chimera binds with an EC50 of 6 - 24 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Disulfide-linked homodimer, biotinylated via amines
Function
B7-2 is a costimulatory molecule that is required for T cell proliferation and IL-2 production.
Ligand Receptor
CD28, CTLA4
Bioactivity
Measured by its ability to bind mouse CTLA4-Fc Chimera.
Cell Sources
Activated antigen presenting cells, including B cells, dendritic cells and macrophages.
Cell Targets
Leukocytes, endothelial cells
Cell Type
B cells, Dendritic cells, Monocytes, NK cells, NKT cells, Th1, Th2
Biology Area
Cell Biology, Costimulatory Molecules, Immunology
Molecular Family
Soluble Receptors
Gene ID
12524 View all products for this Gene ID
UniProt
View information about B7-2/CD86 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 02/13/2019

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