- MP1-31G6 (See other available formats)
- Regulatory Status
- Other Names
- Granulocyte/macrophage-colony stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)
- Rat IgG1, κ
- Ave. Rating
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- Product Citations
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GM-CSF is a hematopoietic factor that is produced by T cells, macrophages, fibroblasts and endothelial cells. This multifunctional cytokine stimulates progenitor cells of neutrophils, eosinophils and macrophages. GM-CSF is also a differentiation and activating factor for granulocytic and monocytic cells. The MP1-31G6 antibody reacts with mouse granulocyte/macrophage-colony stimulating factor (GM-CSF). The MP1-31G6 antibody can neutralize the bioactivity of natural or recombinant GM-CSF.Product Details
- Antibody Type
- Host Species
- Yeast-expressed, recombinant mouse GM-CSF.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
ELISA Detection, ELISPOT Detection
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of GM-CSF recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA or ELISPOT Detection1,3-5: The biotinylated MP1-31G6 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified MP1-22E9 antibody (Cat. No. 505402/505408) as the capture antibody.
Additional reported applications (for the relevant formats) include: Western blotting, immunoprecipitation4, and Neutralization2-4. The LEAF™ purified MP1-22E9 antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse GM-CSF bioactivity in vivo and in vitro (Cat. No. 505408).
(PubMed link indicates BioLegend citation)
- Sander, B., et al. 1993. J. Immunol. Meth. 166:201.
- Suda, T., et al. 1990. Cell. Immunol. 129:228.
- Nozaki, S., et al. 1991. J. Invest. Dermatol. 97:10.
- Abrams, J., et al. 1992. Immunol. Rev. 127:5.
- Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
- Product Citations
AB_315385 (BioLegend Cat. No. 505501)
AB_2085381 (BioLegend Cat. No. 505502)
- Cytokine; 22 kD (Mammalian)
- Growth/development granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
- Cell Sources
- T cells, monocytes/macrophages, fibroblasts, endothelial cells, mast cells
- Cell Targets
- Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
- Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) in common with IL-3R, IL-5R
- Biology Area
- Cell Biology, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Demetri, G., et al. 1991. Blood 78:2791.
3. Fan, D., et al. 1991. In vivo 5:571.
4. Negrin, R., et al. 1992. Adv. Pharmacol. 23:263.
- Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
- Gene ID
- 12981 View all products for this Gene ID
- View information about GM-CSF on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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