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Biotin anti-human CD326 (EpCAM) Antibody

Pricing & Availability
Clone
9C4 (See other available formats)
Regulatory Status
RUO
Other Names
Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, TROP1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
9C4_Biotin_021810
Human colon carcinoma cell line (HT29) stained with biotinylated 9C4, followed by Sav-PE
  • 9C4_Biotin_021810
    Human colon carcinoma cell line (HT29) stained with biotinylated 9C4, followed by Sav-PE
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324215 25 µg £77
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324216 100 µg £166
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Description

CD326 is also known as Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, and TROP1. CD326 is a type I transmembrane protein containing six disulfide bridges and one THYRO domain. This cell surface glycosylated 40 kD protein is highly expressed in bone marrow, colon, lung, and most normal epithelial cells and is expressed on carcinomas of gastrointestinal origin. Recently, it has been reported that CD326 expression occurs during the early steps of erythrogenesis. CD326 functions as a homotypic calcium-independent cell adhesion molecule and is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. CD326 antigen is an immunotherapeutic target for the treatment of human carcinomas.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
DU.4475 breast carcinoma
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the revelant formats) include: immunofluorescence, immunohistochemistry3, and spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Lammers R, et al. 2002. Exp. Hematol. 30:537.
  2. Schultz LD, et al. 2010. P. Natl. Acad. Sci. USA 107:13022. PubMed
  3. Human Protein Atlas http://www.proteinatlas.org/ENSG00000119888/antibody (IHC)
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Civelekoglu O, et al. 2019. Lab Chip. 19:2444. PubMed
  2. Ooi C, et al. 2017. Methods Mol Biol. 10.1007/978-1-4939-7144-2_12. PubMed
  3. Jayasinghe MK, et al. 2022. Theranostics. 12:3288. PubMed
  4. Kathiriya JJ, et al. 2022. Nat Cell Biol. 24:10. PubMed
  5. Zeng Z, et al. 2022. Oncogene. :. PubMed
  6. Kuo C, et al. 2016. Nat Commun. 7: 11468. PubMed
RRID
AB_2231183 (BioLegend Cat. No. 324215)
AB_2262259 (BioLegend Cat. No. 324216)

Antigen Details

Structure
Type I transmembrane protein, contains six disulfide bridges, one THYRO domain, approximate molecular weight 40 kD.
Distribution

Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis.

Function
Homotypic calcium-independent cell adhesion. CD326 is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation.
Modification
Glycosylated.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Strnad J, et al. 1989. Cancer Res. 49:314.
2. Munz M, et al. 2004. Oncogene 23:5748.
3. Rao CG, et al. 2005. Int. J. Oncol. 27:49.

Gene ID
4072 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD326 (EpCAM)

  • FITC anti-human CD326 (EpCAM)

  • PE anti-human CD326 (EpCAM)

  • APC anti-human CD326 (EpCAM)

  • Alexa Fluor® 488 anti-human CD326 (EpCAM)

  • Alexa Fluor® 647 anti-human CD326 (EpCAM)

  • PerCP/Cyanine5.5 anti-human CD326 (EpCAM)

  • Biotin anti-human CD326 (EpCAM)

  • Pacific Blue™ anti-human CD326 (EpCAM)

  • Brilliant Violet 421™ anti-human CD326 (EpCAM)

  • PE/Cyanine7 anti-human CD326 (EpCAM)

  • Brilliant Violet 605™ anti-human CD326 (EpCAM)

  • Brilliant Violet 650™ anti-human CD326 (EpCAM)

  • Alexa Fluor® 594 anti-human CD326 (EpCAM)

  • Purified anti-human CD326 (EpCAM) (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-human CD326 (EpCAM)

  • APC/Fire™ 750 anti-human CD326 (EpCAM)

  • Brilliant Violet 510™ anti-human CD326 (EpCAM)

  • Brilliant Violet 785™ anti-human CD326 (EpCAM)

  • Brilliant Violet 711™ anti-human CD326 (Ep-CAM)

  • TotalSeq™-A0123 anti-human CD326 (Ep-CAM)

  • APC/Cyanine7 anti-human CD326 (Ep-CAM)

  • Alexa Fluor® 700 anti-human CD326 (EpCAM)

  • TotalSeq™-C0123 anti-human CD326 (Ep-CAM)

  • TotalSeq™-B0123 anti-human CD326 (Ep-CAM)

  • PE/Cyanine5 anti-human CD326 (Ep-CAM)

  • TotalSeq™-D0123 anti-human CD326 (Ep-CAM)

  • Spark UV™ 387 anti-human CD326 (Ep-CAM)
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