APC anti-mouse CD31 Antibody

Pricing & Availability
Clone
390 (See other available formats)
Regulatory Status
RUO
Other Names
PECAM-1, EndoCAM
Isotype
Rat IgG2a, κ
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Product Citations
publications
390_APC_072808.jpg
C57BL/6 splenocytes stained with 390 APC
  • 390_APC_072808.jpg
    C57BL/6 splenocytes stained with 390 APC
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102409 25 µg £78
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102410 100 µg £219
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Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).

Application References

(PubMed link indicates BioLegend citation)
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Hudson WH, et al. 2020. Immunity. 51(6):1043-1058.e4.. PubMed
  2. Yang D, et al. 2022. Cell. 185:1905. PubMed
  3. Gao Q, et al. 2019. J Exp Med. 216:688. PubMed
  4. Wang J, et al. 2019. Front Immunol. 9:3157. PubMed
  5. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  6. Cen B, et al. 2021. Oncogene. 40:5984. PubMed
  7. Park M, et al. 2021. BMB Rep. 54:470. PubMed
  8. Yeo S, et al. 2016. Cancer Res . 76: 3397 - 3410. PubMed
  9. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  10. Srivastava N, et al. 2020. FASEB J. 34:12805. PubMed
  11. Hesse J, et al. 2021. eLife. 10:00. PubMed
  12. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  13. Pal B, et al. 2017. Nat Commun.. 10.1038/s41467-017-01560-x. PubMed
  14. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  15. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
  16. Quintana E, et al. 2012. Sci Transl Med. 149:159. PubMed
  17. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  18. Eskiocak U, et al. 2016. Nat Commun. 7:12336. PubMed
  19. Zeng Z, et al. 2022. Oncogene. :. PubMed
  20. Chu X, et al. 2021. Front Cell Dev Biol. 9:671841. PubMed
  21. Sarvi S et al. 2018. Cell chemical biology. 25(12):1456-1469 . PubMed
  22. Long JT, et al. 2022. Elife. 11:. PubMed
  23. Zhang Z, et al. 2021. Exp Dermatol. 30:102. PubMed
  24. Yang T, et al. 2022. Antioxidants (Basel). 11:. PubMed
  25. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  26. Carmody C et al. 2019. Endocrinology. 160(5):1205-1222 . PubMed
  27. Fu NY, et al. 2018. Dev Cell. 47:629. PubMed
  28. Yeo SK, et al. 2020. eLife. 9:e58810.. PubMed
  29. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  30. Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed
  31. Santini MP, et al. 2020. Cell Rep. 30:555. PubMed
  32. Wang X, et al. 2018. Nat Commun. 9:551. PubMed
  33. Hendee K, et al. 2021. Aging (Albany NY). 13:7781. PubMed
  34. Shen B, et al. 2019. Elife. 8:e42274. PubMed
  35. Kalinina A, et al. 2022. PLoS One. 17:e0250752. PubMed
  36. Phansalkar R, et al. 2021. Elife. 10:. PubMed
  37. Kim KS, et al. 2022. JCI Insight. 7:. PubMed
  38. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  39. DeVilbiss AW, et al. 2021. eLife. 10:00. PubMed
  40. Ouadah Y, et al. 2019. Cell. 179:403. PubMed
  41. Nagatake T, et al. 2021. Int Immunol. 33:171. PubMed
  42. Pal B, et al. 2021. Breast Cancer Res. 23:69. PubMed
  43. Dumm RE, et al. 2020. Cell Reports. 32(9):108103.. PubMed
  44. Longchamp A, et al. 2018. Cell. 173:117. PubMed
RRID
AB_312904 (BioLegend Cat. No. 102409)
AB_312905 (BioLegend Cat. No. 102410)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07/19/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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