APC anti-human CD184 (CXCR4) Antibody

Pricing & Availability
12G5 (See other available formats)
Regulatory Status
VII 70204
Other Names
CXCR4, Fusin
Mouse IgG2a, κ
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Product Citations
Human peripheral blood lymphocytes stained with 12G5 APC
  • 12G5_APC_071607
    Human peripheral blood lymphocytes stained with 12G5 APC
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306509 25 tests £106
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306510 100 tests £222
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CD184, also known as fusin or CXCR4, is a 45 kD seven transmembrane G-protein-linked CXC chemokine receptor. CD184 is widely expressed on blood and tissue cells, including B and T cells, monocytes, macrophages, dendritic cells, granulocytes, megakaryocytes/platelets, lymphoid, myeloid precursor cells, endothelial cells, epithelial cells, astrocytes, and neurons, among other tissue cells. CD184 is the receptor for CXC chemokine SDF-1, mediates blood cell migration, and is involved in B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, and cerebellar development. CXCR4 is also a coreceptor of X4 HIV-1 and an alternative receptor for some isolates of HIV-2.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Chimpanzee, Sooty Mangabey
Antibody Type
Host Species
CP-MAC-infected Sup-T1 cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections11, immunocytochemistry3, immunofluorescence microscopy2,6, and blocking of CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-14,5. Clone 12G5 may not be suitable for Western blotting.10 The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 306539 & 306540).

Application References

(PubMed link indicates BioLegend citation)
  1. McKnight A, et al. 1997. J. Virol. 71:1692.
  2. Endres MJ, et al. 1996. Cell 87:745. (Immunogen, IF)
  3. Volin MV, et al. 1998. Biochem. Biophys. Res. Commun. 242:46. (ICC)
  4. Berndt C, et al. 1998. P. Natl. Acad. Sci. USA 95:12556. (Block)
  5. Ullrich CK, et al. 2000. Blood 96:1438. (Block)
  6. Murga M, et al. 2005. Blood 105:1992. (IF)
  7. Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) PubMed
  8. Isnardi I, et al. 2010. Blood 115:5026. PubMed
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  10. Fischer T, et al. 2008. PLoS One 3:e4069.
  11. Schmid BC, et al. 2004. Breast Cancer Res. Treat. 84:247. (IHC)
Product Citations
  1. Gao Y, et al. 2016. Nat Methods. 10.1038/nmeth.4042. PubMed
  2. Biasci D, et al. 2020. Proc Natl Acad Sci U S A. 117:28960. PubMed
  3. Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed
  4. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  5. Xu X, et al. 2021. Molecular Cell. :. PubMed
  6. Isnardi I, et al. 2010. Blood. 115:5026. PubMed
  7. Gludish DW, et al. 2020. Sci Rep. 10:19900. PubMed
  8. Jayasinghe MK, et al. 2022. Theranostics. 12:3288. PubMed
  9. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  10. Roukens AHE, et al. 2022. Nat Immunol. 23:23. PubMed
  11. McQuade A, et al. 2020. Nat Commun. 4.1875. PubMed
  12. Agha O, et al. 2020. JOR Spine. 3:e1091. PubMed
  13. Ngamsri KC, et al. 2021. Int J Mol Sci. 22:. PubMed
  14. Lu P, et al. 2021. Blood Cancer J. 11:39. PubMed
  15. Hyun Hee Lee, Vanessa Bellat, Benedict Law 2017. PLoS One. 12(2):e0171044. PubMed
  16. Huijbregts R, et al. 2013. Endocrinology. 154:1282. PubMed
  17. Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed
  18. Cai J, et al. 2021. eLife. 10:00. PubMed
  19. Rai MA, et al. 2020. Retrovirology. 17:35. PubMed
  20. Kempton HR, et al. 2020. Mol Cell. 184:78. PubMed
  21. Fiedorowicz M, et al. 2020. Sci Rep. 10:5379. PubMed
  22. Foight GW, et al. 2019. Nat Biotechnol. 37:1209. PubMed
  23. Bourges C, et al. 2020. EMBO Mol Med. 12:e12112. PubMed
  24. Suzuki S, et al. 2021. STAR Protocols. 2:100683. PubMed
  25. Toyoda M, et al. 2015. J Virol. 89: 9639-9652. PubMed
  26. Shi J, et al. 2012. Blood. 120:1624. PubMed
  27. Walker KL, et al. 2021. Leuk Lymphoma. 62:1167. PubMed
  28. Meng YM, et al. 2018. Oncoimmunology. 7:e1408745. PubMed
  29. Grosche L, et al. 2020. Viruses. 12:. PubMed
  30. Blanco-Camarillo C, et al. 2021. Front Immunol. 12:672520. PubMed
  31. Ogaki S, et al. 2015. Sci Rep. 5:17297. PubMed
  32. Nakamura M, et al. 2019. Nat Commun. 10:194. PubMed
  33. Corgnac S, et al. 2022. J Immunother Cancer. 10:. PubMed
  34. Zhang Y et al. 2018. Cell stem cell. 23(4):516-529 . PubMed
  35. Rodrigues Toste de Carvalho AL, et al. 2021. Nat Protoc. 16:1802. PubMed
  36. Olety B, et al. 2021. Sci Adv. 7:eabj7398. PubMed
  37. Samuel RM, et al. 2020. Cell Stem Cell. 27:876. PubMed
  38. Shan L, et al. 2017. Immunity. 47:766. PubMed
AB_314615 (BioLegend Cat. No. 306509)
AB_314616 (BioLegend Cat. No. 306510)

Antigen Details

Rhodopsin family, G-protein linked seven transmembrane glycoprotein, 45 kD

T cells and B cells, dendritic cells, monocytes, granulocytes, hematopoietic progenitors, endothelial cells

B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, cerebellar development
SDF-1 receptor, coreceptor for X4 HIV-1
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Monocytes, Neural Stem Cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Berger E, et al. 1999. Annu. Rev. Immunol. 17:657.
2. Loetscher P, et al. 2000. Adv. Immunol. 74:127.
3. Murphy P, et al. 2000. Pharmacol. Rev. 52:145.

Gene ID
7852 View all products for this Gene ID
View information about CD184 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 4    Revision Date: 08/22/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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