APC anti-human CD105 Antibody

Pricing & Availability
Clone
43A3 (See other available formats)
Regulatory Status
RUO
Other Names
Endoglin
Isotype
Mouse IgG1, κ
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Product Citations
publications
43A3_APC_050707
Human monocytic cell line THP-1 stained with 43A3 APC
  • 43A3_APC_050707
    Human monocytic cell line THP-1 stained with 43A3 APC
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323207 25 tests £102
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323208 100 tests £215
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Description

CD105 is also known as Endoglin. It is a type I integral membrane homodimer protein with subunits of 90 kD found on vascular endothelial cells and syncytiotrophoblasts of placenta. CD105 is weakly expressed on stromal fibroblasts. It is also expressed on activated monocytes and tissue macrophages. Expression of CD105 is increased on activated endothelium in tissues undergoing angiogenesis, such as in tumors, or in cases of wound healing or dermal inflammation. CD105 is a component of the TGF-β receptor system in human umbilical vein endothelial cells and binds TGF-β1 and β3 with high affinity but does not bind to TGF-β2.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
L-cells transfected with human CD105
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Bühring HJ, et al. 1991. Leukemia 5:841.
  2. Vogel W, et al. 2002. Haematologica 88:126.
  3. Osaka M, et al. 2011. Brain Res. 9:1343. PubMed
  4. HonMou O, et al. 2011. Brain. 134:1790. PubMed
  5. Herrera MB, et al. 2013. Hepatology. 57:311. PubMed
  6. Iohara K, et al. 2014. Exp Gerontol. 52:39. PubMed
Product Citations
  1. Laviola L, et al. 2012. Endocrinology. 153:5770. PubMed
  2. Radhakrishnan S, et al. 2019. Mol Med Rep. 20:813. PubMed
  3. Jiang S, et al. 2021. Bioact Mater. 2711:6. PubMed
  4. Zhang Z, et al. 2021. Cell Death Dis. 12:644. PubMed
  5. Han J, et al. 2022. FEBS J. 289:417. PubMed
  6. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  7. Gao P, et al. 2017. Cytotechnology. 69:751. PubMed
  8. Li J, et al. 2021. Mol Med Rep. 1:23. PubMed
  9. Di Stefano B, et al. 2019. Cell Stem Cell. 25:622. PubMed
  10. Nelson L, et al. 2020. Nat Commun. 1.029166667. PubMed
  11. Okamura G, et al. 2020. Int J Mol Sci. 22:00. PubMed
  12. Sielatycka K, et al. 2017. Stem Cell Rev. 1.079166667. PubMed
  13. Liu T, et al. 2019. Front Endocrinol (Lausanne). 10:360. PubMed
  14. Gu S, et al. 2016. Sci Rep. 6:23287. PubMed
  15. Thiel A, et al. 2015. Sci Rep. 5: 17685. PubMed
  16. Chijimatsu R, et al. 2022. Regen Ther. 21:52. PubMed
RRID
AB_755959 (BioLegend Cat. No. 323207)
AB_755960 (BioLegend Cat. No. 323208)

Antigen Details

Structure
Associates with TGF-ß receptor I and II, homodimeric type I integral membrane protein, 90 kD
Distribution

Endothelial cells, activated monocytes/macrophages, bone marrow stromal cells, hematopoietic stem/progenitor cells

Function
Modulates cellular response to TGF-ß, involved in vascular development and remodelling
Ligand/Receptor
TGF-ß1, TGF-ß3
Cell Type
Endothelial cells, Hematopoietic stem and progenitors, Macrophages, Mesenchymal Stem Cells, Monocytes
Biology Area
Angiogenesis, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York.
2. Pierelli L, et al. 2001. Leuk. Lymphoma 42:1195.

Gene ID
2022 View all products for this Gene ID
UniProt
View information about CD105 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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