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Alexa Fluor® 700 anti-human CD20 Antibody

Pricing & Availability
Clone
2H7 (See other available formats)
Regulatory Status
RUO
Workshop
IV B201
Other Names
B1, Bp35
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
2H7_Alx700_022008
Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) Alexa Fluor® 700 (filled histogram), or mouse IgG2b, κ Alexa Fluor® 700 (open histogram).
  • 2H7_Alx700_022008
    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) Alexa Fluor® 700 (filled histogram), or mouse IgG2b, κ Alexa Fluor® 700 (open histogram).
Compare all formats See Alexa Fluor® 700 spectral data
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302322 100 µg £182
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Description

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human tonsillar B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation4 and immunohistochemical staining of acetone-fixed frozen sections5.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
  4. Polyak MJ, et al. 2002. Blood 99:3256. (IP)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
Product Citations
  1. Basu M, et al. 2020. Cell Rep Med. :1. PubMed
  2. Schmitz T, et al. 2021. J Reprod Immunol. 148:103424. PubMed
  3. Wang LT, et al. 2020. Immunity. 53:733. PubMed
  4. Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
  5. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  6. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  7. Pandey M, et al. 2021. Clin Transl Immunology. 10:e1260. PubMed
  8. Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
  9. Seydoux E, et al. 2021. Cell Reports. 35(5):109084. PubMed
  10. Weinfurter J, et al. 2011. PLoS One. 6:e1002381. PubMed
  11. Wang C, et al. 2020. Oncologist. 25:382. PubMed
  12. Harris M, et al. 2013. J Virol. 87:9353. PubMed
  13. Kerkman P, et al. 2016. Ann Rheum Dis. 75: 1170 - 1176. PubMed
  14. Piepenbrink M, et al. 2016. PLoS One. 11: 0158641. PubMed
  15. Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
RRID
AB_493753 (BioLegend Cat. No. 302322)

Antigen Details

Structure
Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
Distribution

B cell, T cell subsets

Function
B cell activation
Ligand/Receptor
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Cell Type
B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Hultin L, et al. 1993. Cytometry 14:196.
2. Tedder T, et al. 1994. Immunol. Today 15:450.

Gene ID
931 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org

Related FAQs

What is the difference between two anti human CD20 clones 2H7 and 1412?

For clone 1412: This clone specifically recognizes cytoplasmic domain of CD20 and thus can only be used for intracellular flow cytometry. In this instance you will need to include the fixation and permeabilization steps. Please follow the intracellular flow cytometry staining protocol.

For clone 2H7: This clone is ok for regular surface staining for CD20 and there is no need for any fixation and permeabilization steps.

Our technical protocols can be found here.

Other Formats

View All CD20 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD20 2H7 FC
FITC anti-human CD20 2H7 FC
PE anti-human CD20 2H7 FC
PE/Cyanine5 anti-human CD20 2H7 FC
Purified anti-human CD20 2H7 FC,CyTOF®,IHC-F,IP
APC/Cyanine7 anti-human CD20 2H7 FC
PE/Cyanine7 anti-human CD20 2H7 FC
Alexa Fluor® 488 anti-human CD20 2H7 FC,IHC-F
Alexa Fluor® 647 anti-human CD20 2H7 FC,IHC-F
Pacific Blue™ anti-human CD20 2H7 FC
Alexa Fluor® 700 anti-human CD20 2H7 FC
PerCP anti-human CD20 2H7 FC
PerCP/Cyanine5.5 anti-human CD20 2H7 FC
Brilliant Violet 421™ anti-human CD20 2H7 FC,IHC-F
Brilliant Violet 570™ anti-human CD20 2H7 FC
Brilliant Violet 605™ anti-human CD20 2H7 FC
Brilliant Violet 650™ anti-human CD20 2H7 FC
Brilliant Violet 785™ anti-human CD20 2H7 FC
Brilliant Violet 510™ anti-human CD20 2H7 FC
Brilliant Violet 711™ anti-human CD20 2H7 FC
Purified anti-human CD20 (Maxpar® Ready) 2H7 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD20 2H7 FC
Biotin anti-human CD20 2H7 FC
APC/Fire™ 750 anti-human CD20 2H7 FC
Alexa Fluor® 594 anti-human CD20 2H7 ICC,IHC-F
FITC anti-human CD20 2H7 FC
Pacific Blue™ anti-human CD20 2H7 FC
APC anti-human CD20 2H7 FC
PE/Cyanine7 anti-human CD20 2H7 FC
TotalSeq™-A0100 anti-human CD20 2H7 PG
TotalSeq™-B0100 anti-human CD20 2H7 PG
TotalSeq™-C0100 anti-human CD20 2H7 PG
PerCP/Cyanine5.5 anti-human CD20 2H7 FC
Spark NIR™ 685 anti-human CD20 2H7 FC
Spark YG™ 593 anti-human CD20 2H7 FC
GMP FITC anti-human CD20 2H7 FC
TotalSeq™-D0100 anti-human CD20 2H7 PG
GMP APC anti-human CD20 2H7 FC
Spark Violet™ 500 anti-human CD20 2H7 FC
GMP Pacific Blue™ anti-human CD20 2H7 FC
GMP PerCP/Cyanine5.5 anti-human CD20 2H7 FC
Spark Violet™ 538 anti-human CD20 2H7 FC
Go To Top Version: 4    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD20

  • FITC anti-human CD20

  • PE anti-human CD20

  • PE/Cyanine5 anti-human CD20

  • Purified anti-human CD20

  • APC/Cyanine7 anti-human CD20

  • PE/Cyanine7 anti-human CD20

  • Alexa Fluor® 488 anti-human CD20

  • Alexa Fluor® 647 anti-human CD20

  • Pacific Blue™ anti-human CD20

  • Alexa Fluor® 700 anti-human CD20

  • PerCP anti-human CD20

  • PerCP/Cyanine5.5 anti-human CD20

  • Brilliant Violet 421™ anti-human CD20

  • Brilliant Violet 570™ anti-human CD20

  • Brilliant Violet 605™ anti-human CD20

  • Brilliant Violet 650™ anti-human CD20

  • Brilliant Violet 785™ anti-human CD20

  • Brilliant Violet 510™ anti-human CD20

  • Brilliant Violet 711™ anti-human CD20

  • Purified anti-human CD20 (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-human CD20

  • Biotin anti-human CD20

  • APC/Fire™ 750 anti-human CD20

  • Alexa Fluor® 594 anti-human CD20

  • FITC anti-human CD20

  • Pacific Blue™ anti-human CD20

  • APC anti-human CD20

  • PE/Cyanine7 anti-human CD20

  • TotalSeq™-A0100 anti-human CD20

  • TotalSeq™-B0100 anti-human CD20

  • TotalSeq™-C0100 anti-human CD20

  • PerCP/Cyanine5.5 anti-human CD20

  • Spark NIR™ 685 anti-human CD20

  • Spark YG™ 593 anti-human CD20

  • GMP FITC anti-human CD20

  • TotalSeq™-D0100 anti-human CD20

  • GMP APC anti-human CD20

  • Spark Violet™ 500 anti-human CD20

  • GMP Pacific Blue™ anti-human CD20

  • GMP PerCP/Cyanine5.5 anti-human CD20

  • Spark Violet™ 538 anti-human CD20
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