Alexa Fluor® 647 anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
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Product Citations
publications
1A8_A647_Ly6G_Antibody_1_FC_051816
C57BL/6 mouse bone marrow cells stained with 1A8 Alexa Fluor® 647
  • 1A8_A647_Ly6G_Antibody_1_FC_051816
    C57BL/6 mouse bone marrow cells stained with 1A8 Alexa Fluor® 647
  • 1A8_A647_Ly6G_Antibody_2_IHCF_051816
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 5 µg/mL anti-mouse Ly-6G (clone 1A8) Alexa Fluor® 647 (red), anti-mouse/human CD45R/B220 (clone RA3-6B2) Brilliant Violet 510™ (green) and CD8a (clone 53-6.7) Brilliant Violet 421™ (blue) overnight at 4°C. The image was captured with a 10X objective.
See Alexa Fluor® 647 spectral data See high resolution IHC data...
Cat # Size Price Quantity Avail. Save
127609 25 µg £72
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127610 100 µg £154
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, and depletion4, 12-14. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
Product Citations
  1. Chang T, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006485. PubMed
  2. Li S, et al. 2018. Sci Rep. 8:11247. PubMed
  3. Chung L et al. 2018. Cell host & microbe. 23(2):203-214 . PubMed
  4. Girbl T et al. 2018. Immunity. 49(6):1062-1076 . PubMed
  5. Geng S, et al. 2019. Sci Adv. 5:eaav2309. PubMed
  6. Gall MG, et al. 2019. Sci Rep. 9:7292. PubMed
  7. Chojnacki A, et al. 2019. Commun Biol. 2:181. PubMed
  8. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  9. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  10. Thanabalasuriar A et al. 2019. Cell Host Microbe. 25(4):526-536 . PubMed
  11. Li H et al. 2019. Br J Pharmacol. 176(13):2209-2226 . PubMed
  12. Furuhashi K, et al. 2017. Immunology. 151:227. PubMed
  13. Miyatake Y, et al. 2018. J Immunol. 201:3051. PubMed
  14. Li S, et al. 2019. Front Neurol. 1.036805556. PubMed
  15. Shahid M, et al. 2020. Sci Rep. 0.901388889. PubMed
  16. Wu N, et al. 2020. Cell Rep. 30:1129. PubMed
  17. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  18. Ueki H, et al. 2020. Nat Protoc. 15:1041. PubMed
  19. Shahid M, et al. 2020. Front Cell Infect Microbiol. 10:31. PubMed
  20. Shin JW, et al. 2020. Cell Death Dis. 0.667361111. PubMed
  21. John S, et al. 2020. Sci Rep. 10:11377. PubMed
  22. Ge C, et al. 2020. Cell Reports. 29(13):4236-4244.e3.. PubMed
  23. Tsourouktsoglou TD, et al. 2020. Cell Reports. 31(5):107602. PubMed
  24. Lin R, et al. 2020. Sci Rep. 10:14397. PubMed
  25. Prates D, et al. 2011. J Leukoc Biol. 90:575. PubMed
  26. Li J, et al. 2014. Infect Immun . 82:1579. PubMed
  27. Martinod K, et al. 2015. Blood. 125:1948. PubMed
  28. Meunier I, et al. 2015. PLoS One. 10: 0138055. PubMed
  29. Jhunjhunwala S, et al. 2015. PLoS One. 10: 0137550. PubMed
  30. Traboulsi H, et al. 2015. Antimicrob Agents Chemother. 59: 6317. PubMed
  31. Velázquez F, et al. 2016. J Immunol. 196: 1305 - 1316. PubMed
  32. Katkar G, et al. 2016. Nat Commun. 7: 11361. PubMed
  33. Orberg E, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.53. PubMed
  34. Kretschmer S, et al. 2016. Lab Invest. 10.1038/labinvest.2016.69. PubMed
  35. Bossaller L, et al. 2016. J Immunol. 197: 1044 - 1053. PubMed
  36. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  37. K K, et al. 2016. Haematologica. 151159. PubMed
  38. Kolawole A, et al. 2015. J Virol. 90: 1499-1506. PubMed
  39. Geng S, et al. 2016. Nat Commun. 7:13436. PubMed
  40. Jyh Liang Hor, William R. Heath, Scott N. Mueller 2017. Sci Rep. 7:41091. PubMed
RRID
AB_1134162 (BioLegend Cat. No. 127609)
AB_1134159 (BioLegend Cat. No. 127610)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 01/06/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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